O-GlcNAc Transferase p110 subunit Antibody (678) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43590

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, CyTOF-reported

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 678

Format

Azide and BSA Free
View all O-GlcNAc Transferase/OGT Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human O-GlcNAc Transferase p110 subunit. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

117 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for O-GlcNAc Transferase p110 subunit Antibody (678) - Azide and BSA Free

Western Blot: O-GlcNAc Transferase p110 subunit Antibody (678) [NBP2-43590]

Western Blot: O-GlcNAc Transferase p110 subunit Antibody (678) [NBP2-43590]

Western Blot: O-GlcNAc Transferase p110 subunit Antibody (678) [NBP2-43590] - Analysis of 50 ug mouse brain lysate/extract by 7.5% SDS-PAGE. O-GlcNAc transferase antibody [6G8] at 1:500.
Western Blot: O-GlcNAc Transferase p110 subunit Antibody (678) [NBP2-43590]

Western Blot: O-GlcNAc Transferase p110 subunit Antibody (678) [NBP2-43590]

Western Blot: O-GlcNAc Transferase p110 subunit Antibody (678) [NBP2-43590] - Analysis of 30 ug PC-12 whole cell lysate/extract by 7.5% SDS-PAGE. O-GlcNAc transferase antibody [6G8] at 1:500.
CyTOF-reported: O-GlcNAc Transferase p110 subunit Antibody (678) [NBP2-43590] - Mouse spleen cells were analyzed using NBP2-43590-167Er at 1:100. CyTOF image submitted by a verified customer review.
O-GlcNAc Transferase p110 subunit Antibody (678)

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody (678) [NBP2-43590] -

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody (678) [NBP2-43590] - O-GlcNAc Transferase p110 subunit antibody [GT678] detects O-GlcNAc Transferase p110 subunit protein at cytoplasm of islet by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
O-GlcNAc Transferase p110 subunit stained by O-GlcNAc Transferase p110 subunit antibody [GT678] (NBP2-43590) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
O-GlcNAc Transferase p110 subunit Antibody (678) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody (678) [NBP2-43590] -

O-GlcNAc transferase antibody [GT678] detects O-GlcNAc transferase protein at nucleus (mainly) and cytoplasm (less) by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: O-GlcNAc transferase protein stained by O-GlcNAc transferase antibody [GT678] (NBP2-43590) diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for O-GlcNAc Transferase p110 subunit Antibody (678) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:1000

Western Blot

1:500 - 1:3000
Application Notes
This O-GlcNAc Transferase p110 subunit Antibody (678) is validated for CyTOF from a verified customer review.

Reviewed Applications

Read 1 review rated 4 using NBP2-43590 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: O-GlcNAc Transferase/OGT

Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC's. NPC's contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import.

Long Name

O-Linked N-Acetylglucosamine (GlcNAc) Transferase

Alternate Names

HRNT1, OGlcNAc Transferase, OGT

Gene Symbol

OGT

Additional O-GlcNAc Transferase/OGT Products

Product Documents for O-GlcNAc Transferase p110 subunit Antibody (678) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for O-GlcNAc Transferase p110 subunit Antibody (678) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glycobiology-related Enzymes

Customer Reviews for O-GlcNAc Transferase p110 subunit Antibody (678) - Azide and BSA Free (1)

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  • O-GlcNAc Transferase p110 subunit Antibody (678)
    Name: Zhiqing Li
    Application: CyTOF
    Sample Tested: Mouse spleen cells
    Species: Mouse
    Verified Customer | Posted 03/08/2021
    The spleen cells were analyzed using NBP2-43590-167Er（dilution 1:100）by Cytof.
    O-GlcNAc Transferase p110 subunit Antibody (678) - Azide and BSA Free NBP2-43590
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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