O-GlcNAc Transferase p110 subunit Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89844

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all O-GlcNAc Transferase/OGT Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NMFPHLKKKAVIDFKSNGHIYDNRIVLNGIDLKAFLDSLPDVKIVKMKCPDGGDNADSSNTALNMPVIPMNTIAEAVIEMINRGQIQITINGFSISNGLATT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for O-GlcNAc Transferase p110 subunit Antibody - BSA Free

O-GlcNAc Transferase p110 subunit Antibody - BSA Free Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody - BSA Free [NBP1-89844]

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody - BSA Free [NBP1-89844]

Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
O-GlcNAc Transferase p110 subunit Antibody - BSA Free Western Blot: O-GlcNAc Transferase p110 subunit Antibody - BSA Free [NBP1-89844]

Western Blot: O-GlcNAc Transferase p110 subunit Antibody - BSA Free [NBP1-89844]

Analysis in human cell line HeLa.

Applications for O-GlcNAc Transferase p110 subunit Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: O-GlcNAc Transferase/OGT

Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC's. NPC's contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import.

Long Name

O-Linked N-Acetylglucosamine (GlcNAc) Transferase

Alternate Names

HRNT1, OGlcNAc Transferase, OGT

Gene Symbol

OGT

Additional O-GlcNAc Transferase/OGT Products

Product Documents for O-GlcNAc Transferase p110 subunit Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for O-GlcNAc Transferase p110 subunit Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glycobiology-related Enzymes

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Protocols

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FAQs

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