O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05)

Novus Biologicals | Catalog # NBP2-76848

Recombinant Monoclonal Antibody.
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JG40-05 expressed in HEK293
View all O-GlcNAcase/OGA Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human O-GlcNAcase/OGA/MGEA5 aa 2-103 / 916. (SwissProt: O60502 Human; SwissProt: Q9EQQ9 Mouse)

Localization

Cytoplasm. Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

103 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05) (NBP2-76848) is a recombinant monoclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05)

Western Blot: O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05) [NBP2-76848]

Western Blot: O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05) [NBP2-76848]

Western Blot: O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05) [NBP2-76848] - Western blot analysis of MGEA5 on PC-3M and SH-SY-5Y cell lysates using anti-MGEA5 antibody at 1/500 dilution.
Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05) [NBP2-76848]

Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05) [NBP2-76848]

Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05) [NBP2-76848] - Analysis of mouse testis tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05) [NBP2-76848]

Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05) [NBP2-76848]

Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05) [NBP2-76848] - Analysis of human tonsil tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05) [NBP2-76848]

Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05) [NBP2-76848]

Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05) [NBP2-76848] - Analysis of human pancreas tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.

Applications for O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1:10-1:50

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: O-GlcNAcase/OGA

The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM]

Alternate Names

GH84, HEXC3, Hexosaminidase B, MGEA5, NCOAT, OGA, OGlcNAcase

Gene Symbol

OGA

Additional O-GlcNAcase/OGA Products

Product Documents for O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05)

Certificate of Analysis

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Product Specific Notices for O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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