O-Propargyl-puromycin

Tocris Bioscience | Catalog # 7391

Puromycin analog for labeling newly synthesized proteins
Tocris Bioscience

Key Product Details

Description

Puromycin analog for labeling newly synthesized proteins
View all Other Fluorescent Probes »

Product Description

O-Propargyl-puromycin (OP-puro) is a puromycin analog that is incorporated into nascent polypeptides. It is used for the labeling of newly synthesized proteins in cells and whole animals in vivo. O-Propargyl-puromycin can be click-conjugated to fluorescent azides for visualization by fluoresence microscopy. The product does not require methionine-free conditions. It can also be used in flow cytometry.

Optical Data

λem 275 nm
Reactivity Click
Application Fluorescence Microscopy, Flow Cytometry

Spectra Viewer

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Product Specifications for O-Propargyl-puromycin

Molecular Weight

495.54

Formula

C24H29N7O5

Storage

Store at -20°C

Purity

≥97% (HPLC)

Chemical Name

3'-[[(2S)-2-Amino-1-oxo-3-[4-(2-propyn-1-yloxy)phenyl]propyl]amino]-3'-deoxy-N,N-dimethyladenosine

CAS Number

1416561-90-4

PubChem ID

71576433

InChI Key

JXBIGWQNNSJLQK-IYRMOJGWSA-N

SMILES

O[C@@H]1[C@H](NC([C@@H](N)CC2=CC=C(OCC#C)C=C2)=O)[C@@H](CO)O[C@H]1N3C4=NC=NC(N(C)C)=C4N=C3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 49.55 100

Preparing Stock Solutions for O-Propargyl-puromycin

The following data is based on the product molecular weight 495.54.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.02 mL 10.09 mL 20.18 mL
5 mM 0.40 mL 2.02 mL 4.04 mL
10 mM 0.20 mL 1.01 mL 2.02 mL
50 mM 0.04 mL 0.20 mL 0.40 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 4 publications citing the usage of this product.

Product Documents for O-Propargyl-puromycin

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for O-Propargyl-puromycin

For research use only

Citations for O-Propargyl-puromycin

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