Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all OBFC2B Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 161 and 211 of human single-stranded DNA-binding protein 1 using the numbering given in entry NP_076973.1 (GeneID 79035).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for OBFC2B Antibody - BSA Free

Western Blot: OBFC2B Antibody [NBP1-06561]

Western Blot: OBFC2B Antibody [NBP1-06561]

Western Blot: OBFC2B Antibody [NBP1-06561] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB) and 293T (T; 50 ug for WB; 1 mg for IP, 20% of IP loaded) cells. NBP1-06561 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.
Immunohistochemistry: OBFC2B Antibody [NBP1-06561]

Immunohistochemistry: OBFC2B Antibody [NBP1-06561]

Immunohistochemistry: OBFC2B Antibody [NBP1-06561] - Sample: FFPE sections of human colon carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- SSB1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for OBFC2B Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 mcg/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for human tissues. Epitope retrieval with citrate buffer pH6.0 is recommended for mouse tissues

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: OBFC2B

Single-stranded DNA (ssDNA)-binding proteins, such as OBFC2B, are ubiquitous and essential for a variety of DNA metabolic processes, including replication, recombination, and detection and repair of damage (Richard et al., 2008 [PubMed 18449195]).[supplied by OMIM]

Alternate Names

LP3587, MGC2731, oligonucleotide/oligosaccharide-binding fold containing 2B, Oligonucleotide/oligosaccharide-binding fold-containing protein 2B, Sensor of single-strand DNA complex subunit B1, Sensor of ssDNA subunit B1, Single-stranded DNA-binding protein 1, SOSS complex subunit B1, SOSS-B1, SSB1hSSB1

Entrez Gene IDs

79035 (Human)

Gene Symbol

NABP2

UniProt

NP_076973.1 (Human)

Additional OBFC2B Products

Product Documents for OBFC2B Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for OBFC2B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for OBFC2B Antibody - BSA Free

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Protocols

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