Ocean Blue, SE

Tocris Bioscience | Catalog # 6489

Blue fluorescent dye (Reactive group: NHS ester). Application: flow cytometry, fluorescence microscopy
Tocris Bioscience
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Key Product Details

Description

Blue fluorescent dye (Reactive group: NHS ester). Application: flow cytometry, fluorescence microscopy

Wavelength

Excitation = 405 nm, Emission = 455 nm

Product Description

Key information:

Ocean Blue, SE is a blue fluorescent dye supplied with an NHS ester reactive group for the labeling of primary amines.

Application: Flow cytometry, fluorescence microscopy.

Properties and Photophysical Data: Conjugates of this dye are strongly fluorescent even at neutral pH. Excitation and emission maxima (λ) are 405 nM and 455 nM, respectively; quantum yield = 0.78; extinction coefficient = 36,000 M-1cm-1; A280 correction factor = 0.15.

Please see the protocol for further information on protein/antibody labeling and conjugation.

Note: This product is typically prepared in DMSO.

Optical Data

Emission Color Blue
Brightness
λabs 405 nm
λem 455 nm
Extinction Coefficient (ε) 36000 M-1cm-1
Quantum Yield (φ) 0.78
Closest Laser line 405 nm
Reactive Group NHS ester
Reactivity Primary amines
Correction Factor 280 0.15
Application Flow Cytometry, Fluorescence Microscopy

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Product Specifications for Ocean Blue, SE

Molecular Weight

339.20

Formula

C14H7F2NO7

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

3-Carboxy-6,8-difluoro-7-hydroxycoumaryl succinimidyl ester

CAS Number

215868-33-0

PubChem ID

56927770

InChI Key

NZYXABPVYJRICY-UHFFFAOYSA-N

SMILES

OC1=C(C=C2C=C(C(OC2=C1F)=O)C(ON3C(CCC3=O)=O)=O)F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Background References

References are publications that support the biological activity of the product.

Product Documents for Ocean Blue, SE

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Ocean Blue, SE

For research use only

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