ORP150/HSP12A Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-30107

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ORP150/HSP12A Primary Antibodies »

Product Specifications

Immunogen

between 949 and 999

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ORP150/HSP12A Antibody - BSA Free

ORP150/HSP12A Antibody

Immunoprecipitation:ORP150/HSP12A AntibodyNBP3-30107] -

Immunoprecipitation:ORP150/HSP12A AntibodyNBP3-30107] -Detection of human ORP150/HYOU1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ORP150/HYOU1 antibody (NBP3-30107 lot 1) used for IP at 1 µg per reaction. ORP150/HYOU1 was also immunoprecipitated by a second.inst a different epitope of ORP150/HYOU1 (BL19596). For blotting immunoprecipitated ORP150/HYOU1, NBP3-30107 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for ORP150/HSP12A Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ORP150/HSP12A

HYOU1 is encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment is found at the 5' end of the first exon, and is involved in stress-dependent induction. A transcript that is produced from a downstream start site is preferentially induced by hypoxia, resulting in the accumulation of this protein in the endoplasmic reticulum (ER). The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol.

Long Name

Oxygen Regulated Protein, 150 kDa

Alternate Names

HSP12A, HYOU1

Gene Symbol

HYOU1

Additional ORP150/HSP12A Products

Product Documents for ORP150/HSP12A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ORP150/HSP12A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Heat Shock Proteins (HSPs)

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