p130Cas/BCAR1 Antibody (GT331) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13565

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT331

Format

Azide and BSA Free
View all p130Cas Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human p130Cas/BCAR1. The exact sequence is proprietary.

Reactivity Notes

Mouse (88%), Rat (88%), Bovine (88%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Centrifuge briefly prior to opening.

Scientific Data Images for p130Cas/BCAR1 Antibody (GT331) - Azide and BSA Free

p130Cas/BCAR1 Antibody (GT331)

Western Blot: p130Cas/BCAR1 Antibody (GT331) [NBP3-13565] -

Western Blot: p130Cas/BCAR1 Antibody (GT331) [NBP3-13565] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas/BCAR1 antibody [GT331] (NBP3-13565) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: p130Cas/BCAR1 Antibody (GT331) [NBP3-13565]

Immunohistochemistry-Paraffin: p130Cas/BCAR1 Antibody (GT331) [NBP3-13565]

Immunohistochemistry-Paraffin: p130Cas/BCAR1 Antibody (GT331) [NBP3-13565] - p130Cas/BCAR1 antibody [GT331] detects p130Cas/BCAR1 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. p130Cas/BCAR1 antibody [GT331] (NBP3-13565) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: p130Cas/BCAR1 Antibody (GT331) [NBP3-13565]

Immunohistochemistry-Paraffin: p130Cas/BCAR1 Antibody (GT331) [NBP3-13565]

Immunohistochemistry-Paraffin: p130Cas/BCAR1 Antibody (GT331) [NBP3-13565] - p130Cas/BCAR1 antibody [GT331] detects p130Cas/BCAR1 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. p130Cas/BCAR1 antibody [GT331] (NBP3-13565) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
p130Cas/BCAR1 Antibody (GT331) - Azide and BSA Free

Immunohistochemistry-Frozen: p130Cas/BCAR1 Antibody (GT331) [NBP3-13565] -

p130Cas antibody [GT331] detects p130Cas protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: p130Cas protein stained by p130Cas antibody [GT331] (NBP3-13565) diluted at 1:250.
Blue: Fluoroshield with DAPI.

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Applications for p130Cas/BCAR1 Antibody (GT331) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p130Cas

BCAR1/p130CAS functions as a scaffolding protein for components of various kinase signaling pathways. It is involved in integrin signaling for cell motility as well as netrin signaling for the guidance of commissural axons. Overexpression of BCAR1/p130CAS has been shown to confer antiestrogen resistance in breast cancer cells. Alternate names for BCAR1/p130CAS include breast cancer anti-estrogen resistance protein 1, CRK-associated substrate, CAS scaffolding protein family member 1, CAS, and CRKAS.

Long Name

Breast Cancer Anti-estrogen Resistance 1

Alternate Names

BCAR1, CASS1, CRKAS

Gene Symbol

BCAR1

Additional p130Cas Products

Product Documents for p130Cas/BCAR1 Antibody (GT331) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for p130Cas/BCAR1 Antibody (GT331) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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