p57 Kip2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-28707

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all p57 Kip2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 266 and 316 of human cyclin-dependent kinase inhibitor p57 using the numbering given in entry NP_000067.1 (GeneID 1028).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for p57 Kip2 Antibody - BSA Free

Immunoprecipitation: p57 Kip2 Antibody [NBP1-28707]

Immunoprecipitation: p57 Kip2 Antibody [NBP1-28707]

Immunoprecipitation: p57 Kip2 Antibody [NBP1-28707] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-p57kip2 antibody NBP1-28706 used for IP at 3 ug/mg lysate. p57kip2 was also immunoprecipitated by rabbit anti-p57kip2 antibody NBP1-28707, which recognizes an upstream epitope. For blotting immunoprecipitated p57kip2, NBP1-28706 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for p57 Kip2 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
This antibody is useful for immunoprecipitation. It is not recommended for western analysis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: p57 Kip2

p57kip2 is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate.

Alternate Names

Beckwith-Wiedemann syndrome, BWS, cyclin-dependent kinase inhibitor 1C, cyclin-dependent kinase inhibitor 1C (p57, Kip2), Cyclin-dependent kinase inhibitor p57, KIP2BWCR, p57, p57Kip2, WBS

Entrez Gene IDs

1028 (Human)

Gene Symbol

CDKN1C

UniProt

NP_000067.1 (Human)

Additional p57 Kip2 Products

Product Documents for p57 Kip2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for p57 Kip2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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