PA Janelia Fluor® 549, Maleimide

Tocris Bioscience | Catalog # 8132

Yellow photoactivatable dye (Reactive group: maleimide). Application: flow cytometry, confocal microscopy, super-resolution microscopy including dSTORM & STED. Suitable for live cell imaging
Tocris Bioscience

Key Product Details

Description

Yellow photoactivatable dye (Reactive group: maleimide). Application: flow cytometry, confocal microscopy, super-resolution microscopy including dSTORM & STED. Suitable for live cell imaging
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Product Description

Key information:

PA Janelia Fluor® 549, Maleimide is a yellow cell-permeable photoactivatable fluorescent dye; supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for live and fixed cell imaging.

Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 551 - 553 nm and 570 - 573 nm, respectively.

Please see the product protocol for further information and a guide to protein/antibody labeling.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data

Emission Color Yellow
Closest Laser line 523 nm
Reactive Group Maleimide
Reactivity Thiols
Cell Permeable Yes
Application Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry, dSTORM, STED Microscopy
Alternative For Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3

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Product Specifications for PA Janelia Fluor® 549, Maleimide

Molecular Weight

600.64

Formula

C34H28N6O5

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

3',6'-Di(azetidin-1-yl)-2-diazo-N-(2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)-3-oxo-2,3-dihydrospiro[indene-1,9'-xanthene]-6-carboxamide

InChI Key

GEIGGZSHCKWEPJ-UHFFFAOYSA-N

SMILES

O=C(NCCN1C(C=CC1=O)=O)C2=CC=C(C(C3=[N+]=[N-])=O)C(C43C5=CC=C(N6CCC6)C=C5OC7=CC(N8CCC8)=CC=C47)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 6.01 10

Preparing Stock Solutions for PA Janelia Fluor® 549, Maleimide

The following data is based on the product molecular weight 600.64.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 16.65 mL 83.24 mL 166.49 mL
0.5 mM 3.33 mL 16.65 mL 33.30 mL
1 mM 1.66 mL 8.32 mL 16.65 mL
5 mM 0.33 mL 1.66 mL 3.33 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for PA Janelia Fluor® 549, Maleimide

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PA Janelia Fluor® 549, Maleimide

For research use only

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