PA Janelia Fluor® 646, Maleimide
Tocris Bioscience | Catalog # 8133
Key Product Details
Description
Product Description
Key information: PA Janelia Fluor® 646, Maleimide is a red cell-permeable photoactivatable fluorescent dye; supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for live and fixed cell imaging.Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 649 - 651 nm and 663 - 665 nm, respectively.
Please see the product protocol for further information and a guide to protein/antibody labeling.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
<Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research CampusOptical Data
| Emission Color | Red |
| Closest Laser line | 640 nm |
| Reactive Group | Maleimide |
| Reactivity | Thiols |
| Cell Permeable | Yes |
| Application | Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry, dSTORM, STED Microscopy |
| Alternative For | Alexa Fluor® 647, Atto 647, Atto 647N, CF640R, CF647, Cyanine 5 |
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Product Specifications for PA Janelia Fluor® 646, Maleimide
Molecular Weight
Formula
Storage
Purity
Chemical Name
CAS Number
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 6.43 | 10 |
Preparing Stock Solutions for PA Janelia Fluor® 646, Maleimide
The following data is based on the product molecular weight 642.79.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 15.56 mL | 77.79 mL | 155.57 mL |
| 0.5 mM | 3.11 mL | 15.56 mL | 31.11 mL |
| 1 mM | 1.56 mL | 7.78 mL | 15.56 mL |
| 5 mM | 0.31 mL | 1.56 mL | 3.11 mL |
Calculators
Background References
References are publications that support the biological activity of the product.
- Grimm Bright photoactivatable fluorophores for single-molecule imaging. Nat.Methods 2016 PMID: 27776112
Product Documents for PA Janelia Fluor® 646, Maleimide
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PA Janelia Fluor® 646, Maleimide
For research use only
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