PAF49 Antibody (7212) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-43592
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 7212
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human PAF49. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for PAF49 Antibody (7212) - Azide and BSA Free
Western Blot: PAF49 Antibody (7212) [NBP2-43592]
Western Blot: PAF49 Antibody (7212) [NBP2-43592] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAF49 antibody diluted at 1:500.Western Blot: PAF49 Antibody (7212) [NBP2-43592]
Western Blot: PAF49 Antibody (7212) [NBP2-43592] - Analysis of A. 30 ug 293T whole cell lysate/extract 7.5 % SDS-PAGE PAF49 antibody [7212] dilution: 1:500.Immunocytochemistry/ Immunofluorescence: PAF49 Antibody (7212) [NBP2-43592] -
CD3EAP antibody detects CD3EAP protein at Nucleus by immunofluorescent analysis.Sample: U2OS cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: CD3EAP protein stained by CD3EAP antibody (NBP2-43592) diluted at 1:500.
Blue: Hoechst 33342 staining.
Applications for PAF49 Antibody (7212) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PAF49
Alternate Names
A34.5, antisense to ERCC 1, Antisense to ERCC-1 protein, ASE-1ASE1, CASTMGC118851, CD3 epsilon associated protein, CD3e antigen, epsilon polypeptide associated protein, CD3e molecule, epsilon associated protein, CD3E-associated protein, CD3-epsilon-associated protein, DNA-directed RNA polymerase I subunit RPA34, PAF49RNA polymerase I-associated factor PAF49
Gene Symbol
CD3EAP
Additional PAF49 Products
Product Documents for PAF49 Antibody (7212) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PAF49 Antibody (7212) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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