PALS1/MPP5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-29016

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PALS1/MPP5 Primary Antibodies »

Product Specifications

Immunogen

Between 225 and 275

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PALS1/MPP5 Antibody - BSA Free

PALS1/MPP5 Antibody

Immunoprecipitation:PALS1/MPP5 AntibodyNBP3-29016] -

Immunoprecipitation:PALS1/MPP5 AntibodyNBP3-29016] -Detection of human MPP5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MPP5 antibody NBP3-29016 (lot NBP3-29016-1) used for IP at 6 µg per reaction. MPP5 was also immunoprecipitated by rabbit anti-MPP5 antibody. For blotting immunoprecipitated MPP5, NBP3-29016 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for PALS1/MPP5 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PALS1/MPP5

Members of the peripheral membrane-associated guanylate kinase (MAGUK) family function in tumor suppression and receptor clustering by forming multiprotein complexes containing distinct sets of transmembrane, cytoskeletal, and cytoplasmic signaling proteins. All MAGUKs contain a PDZ-SH3-GUK core and are divided into 4 subfamilies, DLG-like (see DLG1; MIM 601014), ZO1-like (see TJP1; MIM 601009), p55-like (see MPP1; MIM 305360), and LIN2-like (see CASK; MIM 300172), based on their size and the presence of additional domains (Tseng et al., 2001). MPP5 is a member of the p55-like MAGUK subfamily.

Long Name

Protein Associated with Lin Seven 1

Alternate Names

MPP5, Stardust

Gene Symbol

PALS1

Additional PALS1/MPP5 Products

Product Documents for PALS1/MPP5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PALS1/MPP5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epithelial Cell Polarity

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Protocols

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