Biological ActivityPam3CSK4 is a toll-like receptor 1/2 (TLR1/2) agonist; induces production of TNF-α and IL-6 in macrophages. Stimulates phosphorylation of p100/p110 and p60 in granulocytic-differentiated HL-60 cells. Promotes differentiation of naive CD4+ T cells into Th17 cells.
(Modifications: Cys-1 = S-[2,3-Bis(palmitoyloxy)-(2-R/S)-propyl]-N-palmitoyl]-Cys
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Lipopeptides are effective stimulators of tyrosine phosphorylation in human myeloid cells.
Offermanns et al.
MF59 and Pam3CSK4 boost adaptive responses to influenza subunit vaccine through an IFN type I-independent mechanism of action.
Caproni et al.
Toll-like receptor ligands induce the expression of IF.-gamma and interleukin-17 in chicken CD4+ T cells.
St Paul et al.
BMC Res.Notes, 2012;1:616
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We used 50 ng/ml of Pam3CSK4 to treat differentiated human macrophages for 2 h at 37C and measured the IL-6 production in culture supernatants of macrophages. As shown in the attached image, Pam3CSK4 treatment, but not untreated cells, significantly induced IL-6 production in human macrophages.
Raw-Blue Cell lines were treated with 125 ng/ml of PAM3CSK4 and the level of SEAP was measured. SEAP level correlates with NFkB activation
I use this product to activate TLR2