Pancreatic Amylase Alpha Antibody (OTI1E3)

Novus Biologicals | Catalog # NBP2-46518

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1E3
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Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 135-408 of human AMY2A (NP_000690) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

55.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Pancreatic Amylase Alpha Antibody (OTI1E3)

Western Blot: Pancreatic Amylase Alpha Antibody (OTI1E3) [NBP2-46518]

Western Blot: Pancreatic Amylase Alpha Antibody (OTI1E3) [NBP2-46518]

Western Blot: Pancreatic Amylase Alpha Antibody (1E3) [NBP2-46518] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AMY2A.
Immunohistochemistry: Pancreatic Amylase Alpha Antibody (OTI1E3) [NBP2-46518]

Immunohistochemistry: Pancreatic Amylase Alpha Antibody (OTI1E3) [NBP2-46518]

Immunohistochemistry: Pancreatic Amylase Alpha Antibody (1E3) [NBP2-46518] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for Pancreatic Amylase Alpha Antibody (OTI1E3)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Reviewed Applications

Read 2 reviews rated 4 using NBP2-46518 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Pancreatic Amylase Alpha

Alternate Names

AMY2, AMY2A amylase, alpha 2A (pancreatic), AMY2B, PA, pancreatic alpha-amylase

Gene Symbol

AMY2A

Additional Pancreatic Amylase Alpha Products

Product Documents for Pancreatic Amylase Alpha Antibody (OTI1E3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Pancreatic Amylase Alpha Antibody (OTI1E3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Pancreatic Amylase Alpha Antibody (OTI1E3) (2)

4 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
0%
3 Stars
50%
2 Stars
0%
1 Stars
0%

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Customer Images


Showing  1 - 2 of 2 reviews Showing All
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  • Pancreatic Amylase Alpha Antibody (1E3)
    Name: Scott Messenger
    Application: Immunocytochemistry
    Sample Tested: Mouse pancreas in OCT
    Species: Mouse
    Verified Customer | Posted 06/09/2017
    mouse pancreas, 11 um section, 1/200 primary, 1/200 secondardy 568. DAPI labeled nuclei. Unsure if zymogen granules are marked. Epi-Fluorescence 60 x objective
    Pancreatic Amylase Alpha Antibody (OTI1E3) NBP2-46518
  • Pancreatic Amylase Alpha Antibody (1E3)
    Name: Scott Messenger
    Application: Western Blot
    Sample Tested: acini (group of isolated acinar cells) and AR42J rat acinar pancreatic cancer cell line
    Species: Human and Rat
    Verified Customer | Posted 05/05/2017
    human or rat acini, AR42J left untreated or 72 h with dexamethasone. 50 ug whole cell lysate. primary 1/1000
    Pancreatic Amylase Alpha Antibody (OTI1E3) NBP2-46518

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Protocols

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