Recombinant Human PAPSS1 His Protein

Name: Recombinant Human PAPSS1 His Protein
Brand: Novus Biologicals
SKU: NBP1-78889
Price: 419 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-78889

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all PAPSS1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 24-624 of Human PAPSS1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMRATNV TYQAHHVSRN KRGQVVGTRG GFRGCTVWLT GLSGAGKTTV SMALEEYLVC HGIPCYTLDG DNIRQGLNKN LGFSPEDREE NVRRIAEVAK LFADAGLVCI TSFISPYTQD RNNARQIHEG ASLPFFEVFV DAPLHVCEQR DVKGLYKKAR AGEIKGFTGI DSEYEKPEAP ELVLKTDSCD VNDCVQQVVE LLQERDIVPV DASYEVKELY VPENKLHLAK TDAETLPALK INKVDMQWVQ VLAEGWATPL NGFMREREYL QCLHFDCLLD GGVINLSVPI VLTATHEDKE RLDGCTAFAL MYEGRRVAIL RNPEFFEHRK EERCARQWGT TCKNHPYIKM VMEQGDWLIG GDLQVLDRVY WNDGLDQYRL TPTELKQKFK DMNADAVFAF QLRNPVHNGH ALLMQDTHKQ LLERGYRRPV LLLHPLGGWT KDDDVPLMWR MKQHAAVLEE GVLNPETTVV AIFPSPMMYA GPTEVQWHCR ARMVAGANFY IVGRDPAGMP HPETGKDLYE PSHGAKVLTM APGLITLEIV PFRVAAYNKK KKRMDYYDSE HHEDFEFISG TRMRKLAREG QKPPEGFMAP KAWTVLTEYY KSLEKA

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

70.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PAPSS1 His Protein

SDS-PAGE: Recombinant Human PAPSS1 His Protein [NBP1-78889]

SDS-Page: Recombinant Human PAPSS1 Protein [NBP1-78889] - 15% SDS-PAGE (3ug)

Formulation, Preparation, and Storage

NBP1-78889

Formulation	20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PAPSS1

PAPSS1, also known as bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1, is a bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway, yielding 3'-phosphoadenylylsulfate (PAPS). PAPSS1 is also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells. Recombinant human PAPSS1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

3'-phosphoadenosine 5'-phosphosulfate synthase 1, ATPSK1SK 1, bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1, EC 2.7.1.25, PAPS synthase 1, PAPSS 1, PAPSS3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 1, SK1, Sulfurylase kinase 1

Gene Symbol

PAPSS1

Additional PAPSS1 Products

Product Documents for Recombinant Human PAPSS1 His Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human PAPSS1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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