PARP12 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-30089

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Between 300 and 350

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PARP12 Antibody - BSA Free

PARP12 Antibody

Immunoprecipitation:PARP12 AntibodyNBP3-30089] -

Immunoprecipitation:PARP12 AntibodyNBP3-30089] -Detection of human PARP12 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PARP12 antibody NBP3-30089 (lot NBP3-30089-1) used for IP at 6 µg per reaction. For blotting immunoprecipitated PARP12, NBP3-30089 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
PARP12 Antibody

Western Blot:PARP12 AntibodyNBP3-30089] -

Western Blot:PARP12 AntibodyNBP3-30089] -Whole cell lysate (50 µg) from HeLa, HEK293T,Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells preparedusing NETN lysis buffer. Antibody: Affinity purified rabbitanti-PARP12 antibody usedfor WB at 0.1 µg/ml. Detection: Chemiluminescence withan exposure time of 1 second.

Applications for PARP12 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 µg/mg lysate

Western Blot

1:2,000 - 1:10,000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PARP12

Poly (ADP-ribose) polymerase (PARP) catalyzes the post-translational modification of proteins by the addition ofmultiple ADP-ribose moieties. PARP transfers ADP-ribose from nicotinamide dinucleotide (NAD) to glu/asp residues onthe substrate protein, and also polymerizes ADP-ribose to form long/branched chain polymers. PARP inhibitors are beingdeveloped for use in a number of pathologies including cancer, diabetes, stroke and cardiovascular disease.

Long Name

Protein mono-ADP-ribosyltransferase PARP12

Alternate Names

ARTD12, PARP-12, ZC3HDC1

Gene Symbol

PARP12

Additional PARP12 Products

Product Documents for PARP12 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PARP12 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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