Patched 1/PTCH Antibody (PSH03-53)
Novus Biologicals | Catalog # NBP3-32718
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Localization
Clonality
Host
Isotype
Theoretical MW
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Patched 1/PTCH Antibody (PSH03-53)
Western Blot: Patched 1/PTCH Antibody (PSH03-53) [NBP3-32718]
Western Blot: Patched 1/PTCH Antibody (PSH03-53) [NBP3-32718] - Western blot analysis of Patched 1/PTCH on different lysates with Rabbit anti-Patched 1/PTCH antibody (NBP3-32718) at 1/10,000 dilution.Lane 1: 293T-NT cell lysate
Lane 2: 293T-OE-PTCH1 cell lysate
Lysates/proteins at 1 ug/Lane.
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32718) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for Patched 1/PTCH Antibody (PSH03-53)
Western Blot
Formulation, Preparation, and Storage
Purification
Formulation
Preservative
Concentration
Shipping
Stability & Storage
Background: Patched 1/PTCH
Alternate Names
Gene Symbol
Additional Patched 1/PTCH Products
Product Documents for Patched 1/PTCH Antibody (PSH03-53)
Certificate of Analysis
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Product Specific Notices for Patched 1/PTCH Antibody (PSH03-53)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Patched 1/PTCH Antibody (PSH03-53)
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Q: I need a proven anti-human Ptch1 antibody that maps C-terminus of Ptch1 protein which is thus appropriate for all isoforms of human Ptch1 protein. Can you please suggest any which can be used for Western blotting?
A:
NB100-41101 is expected to recognize all isoforms. NBP2-19705, NBP1-71662, 21130002, and NBP1-59455 should all suit your needs as well. Further details about each product can be found on their individual datasheets.