PDF Antibody (OTI3F3)

Novus Biologicals | Catalog # NBP2-00814

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3F3
View all PDF Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PDF (NP_071736) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

19.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDF Antibody (OTI3F3)

Western Blot: PDF Antibody (OTI3F3) [NBP2-00814]

Western Blot: PDF Antibody (OTI3F3) [NBP2-00814]

Western Blot: PDF Antibody (3F3) [NBP2-00814] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDF.
Immunocytochemistry/Immunofluorescence: PDF Antibody (3F3) [NBP2-00814] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PDF.
Flow Cytometry: PDF Antibody (OTI3F3) [NBP2-00814]

Flow Cytometry: PDF Antibody (OTI3F3) [NBP2-00814]

Flow Cytometry: PDF Antibody (3F3) [NBP2-00814] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDF antibody, and then analyzed by flow cytometry.
Flow Cytometry: PDF Antibody (OTI3F3) [NBP2-00814]

Flow Cytometry: PDF Antibody (OTI3F3) [NBP2-00814]

Flow Cytometry: PDF Antibody (3F3) [NBP2-00814] - Analysis of Hela cells, using anti-PDF antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: PDF Antibody (OTI3F3) [NBP2-00814]

Flow Cytometry: PDF Antibody (OTI3F3) [NBP2-00814]

Flow Cytometry: PDF Antibody (3F3) [NBP2-00814] - Analysis of Jurkat cells, using anti-PDF antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for PDF Antibody (OTI3F3)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.78 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PDF

Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells. [provided by RefSeq].

Alternate Names

EC 3.5.1.88, GDF15, MIC1, PDF1A, peptide deformylase (mitochondrial), peptide deformylase, mitochondrial, peptide deformylase-like protein, PLAB, Polypeptide deformylase

Entrez Gene IDs

64146 (Human)

Gene Symbol

PDF

Additional PDF Products

Product Documents for PDF Antibody (OTI3F3)

Certificate of Analysis

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Product Specific Notices for PDF Antibody (OTI3F3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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