PDK2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87307

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Westen Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PDK2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KTSFTFLRQELPVRLANIMKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTN

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25991547).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PDK2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PDK2 Antibody [NBP1-87307]

Immunocytochemistry/ Immunofluorescence: PDK2 Antibody [NBP1-87307]

Immunocytochemistry/Immunofluorescence: PDK2 Antibody [NBP1-87307] - Staining of human cell line A-431 shows localization to nucleoplasm & mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PDK2 Antibody [NBP1-87307]

Immunohistochemistry-Paraffin: PDK2 Antibody [NBP1-87307]

Immunohistochemistry-Paraffin: PDK2 Antibody [NBP1-87307] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.

Applications for PDK2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. WB reported in the literature (PMID: 30457021 and 25991547).

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDK2

PDK2 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism

Long Name

[Pyruvate dehydrogenase

Alternate Names

PDH kinase 2, PDHK2, PDKII

Gene Symbol

PDK2

Additional PDK2 Products

Product Documents for PDK2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PDK2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PDK2 Antibody - BSA Free

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Protocols

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FAQs

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