PFDN5 Antibody (9Y5H7)

Novus Biologicals | Catalog # NBP3-16331

Recombinant Monoclonal Antibody
Novus Biologicals
Loading...

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9Y5H7 expressed in HEK293
Loading...

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-154 of human PFDN5 (Q99471). LNVLNKSNEGKELLVPLTSSMYVPGKLHDVEHVLIDVGTGYYVEKTAEDAKDFFKRKIDFLTKQMEKIQPALQEKHAMKQAVMEMMSQKIQQLTALGAAQATAKA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PFDN5 Antibody (9Y5H7)

Western Blot: PFDN5 Antibody (9Y5H7) [NBP3-16331]

Western Blot: PFDN5 Antibody (9Y5H7) [NBP3-16331]

Western Blot: PFDN5 Antibody (9Y5H7) [NBP3-16331] - Western blot analysis of extracts of various cell lines, using PFDN5 Rabbit mAb (NBP3-16331) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
PFDN5 Antibody (9Y5H7)

Immunocytochemistry/ Immunofluorescence: PFDN5 Antibody (9Y5H7) [NBP3-16331] -

Immunocytochemistry/ Immunofluorescence: PFDN5 Antibody (9Y5H7) [NBP3-16331] - Confocal imaging of HeLa cells using PFDN5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunocytochemistry/ Immunofluorescence: PFDN5 Antibody (9Y5H7) [NBP3-16331]

Immunocytochemistry/ Immunofluorescence: PFDN5 Antibody (9Y5H7) [NBP3-16331]

Immunocytochemistry/Immunofluorescence: PFDN5 Antibody (9Y5H7) [NBP3-16331] - Immunofluorescence analysis of HeLa cells using PFDN5 Rabbit mAb (NBP3-16331) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for PFDN5 Antibody (9Y5H7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PFDN5

PFDN5 encodes a member of the prefoldin alpha subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. The encoded protein may also repress the transcriptional activity of the proto-oncogene c-Myc. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Alternate Names

c-myc binding protein, C-Myc-binding protein Mm-1, MM-1, MM1MGC71907, Myc modulator 1, myc modulator-1, PFD5MGC5329, prefoldin 5, prefoldin subunit 5

Gene Symbol

PFDN5

Additional PFDN5 Products

Product Documents for PFDN5 Antibody (9Y5H7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PFDN5 Antibody (9Y5H7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for PFDN5 Antibody (9Y5H7)

There are currently no reviews for this product. Be the first to review PFDN5 Antibody (9Y5H7) and earn rewards!

Have you used PFDN5 Antibody (9Y5H7)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies
Loading...