Phalloidin-Janelia Fluor® 646

Tocris Bioscience | Catalog # 7201

Red-fluorescent F-actin probe. Binds and labels F-actin. Used for: F-actin labelling. Application: confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED, flow cytometry
Tocris Bioscience
Discontinued Product
7201 has been discontinued. View all Janelia Fluor Dyes products.

Key Product Details

Description

Red-fluorescent F-actin probe. Binds and labels F-actin. Used for: F-actin labelling. Application: confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED, flow cytometry

Alternative Names

Phalloidin-JF646

Wavelength

Excitation = 646 nm, Emission = 664 nm
View all Janelia Fluor Dyes »

Product Description

Key information

: Phalloidin-Janelia Fluor® 646 is a red fluorescent F-actin probe. It binds and labels specifically F-actin.

Used for: Phalloidin-Janelia Fluor® 646 can be used to visualize and quantify F-actin in tissue sections, cell cultures or cell-free experiments with optimal results in formaldehyde-fixed and permeabilized cells.

Application: confocal microscopy, super-resolution microscopy (SRM) including dSTORM and STED and flow cytometry.

Properties and Photophysical Data: Phalloidin-Janelia Fluor® 646 is composed of the F-actin probe, Phalloidin (Cat. No. 4535), conjugated to Janelia Fluor® 646 (Cat. No. 6148). Excitation and emission maxima (λ) are 646 nm and 664 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1.

Scientific Data Images for Phalloidin-Janelia Fluor® 646

Application of Phalloidin-Janelia Fluor® 646

Application of Phalloidin-Janelia Fluor® 646.

HeLa cells were fixed for 10 minutes with 4% formaldehyde in PBS and permeabilized with PBS/0.25% Triton X-100 for 6 minutes. An additional post-fixation step was performed twice with 1% formaldehyde in PBS followed by 3 washes with PBS. Phalloidin Janelia Fluor® 646 was prepared as a 10 μM stock solution diluted in methanol and diluted to a working concentration of 20 nM in PBS. Stained cells were imaged with a Leica SP8X WLL upright confocal microscope. Image kindly provided by Brigitte Bergner, Steffen Dietzel, Core Facility Bioimaging at the Biomedical Center of the Ludwig-Maximilians-Universität München.
Application of Phalloidin-Janelia Fluor® 646

Application of Phalloidin-Janelia Fluor® 646.

HeLa cells were fixed for 10 minutes with 4% formaldehyde in PBS and permeabilized with PBS/0.25% Triton X-100 for 6 minutes. An additional post-fixation step was performed twice with 1% formaldehyde in PBS followed by 3 washes with PBS. Phalloidin Janelia Fluor® 646 was prepared as a 10 μM stock solution diluted in methanol and diluted to a working concentration of 20 nM in PBS. Stained cells were imaged with a Leica SP8X WLL upright confocal microscope. Image kindly provided by Brigitte Bergner, Steffen Dietzel, Core Facility Bioimaging at the Biomedical Center of the Ludwig-Maximilians-Universität München.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data

Phalloidin-Janelia Fluor<sup>®</sup> 646 Dye Spectra
Emission Color Red
Brightness
λabs 646 nm
λem 664 nm
Extinction Coefficient (ε) 152,000 M-1cm-1
Quantum Yield (φ) 0.54
Closest Laser line 640 nm
Application Confocal Imaging, dSTORM, STED, Flow Cytometry

Spectra Viewer

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Product Specifications for Phalloidin-Janelia Fluor® 646

Molecular Weight

1266.51

Formula

C64H75N11O13SSi

Storage

Store at -20°C

Purity

≥90% (HPLC)

InChI Key

AOXMEZGPCIYGOB-BNAQDRCBSA-N

SMILES

O=C([C@H](NC([C@H](C)NC([C@H](C[C@](O)(C)CNC(C1=CC=C(C([O-])=O)C(C2=C(C=C/3)C([Si](C)(C)C4=CC(N5CCC5)=CC=C42)=CC3=[N+]6CCC\6)=C1)=O)N7)=O)=O)[C@H](C)O)N[C@H]8CSC(N9)=C(C[C@H](NC([C@H](C)NC([C@@H]%10C[C@@H](O)CN%10C8=O)=O)=O)C7=O)C%11=C9C=CC=C%11

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Background References

References are publications that support the biological activity of the product.

Product Documents for Phalloidin-Janelia Fluor® 646

Certificate of Analysis

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Product Specific Notices for Phalloidin-Janelia Fluor® 646

For research use only

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