Phorbol 12-myristate 13-acetate

Tocris Bioscience | Catalog # 1201

Protein kinase C activator
Tocris Bioscience

Key Product Details

Description

Protein kinase C activator

Alternative Names

PMA
View all Protein Kinase C Activators »

Product Description

Phorbol 12-myristate 13-acetate is an extensively used phorbol ester high affinity activator of protein kinase C (Ki = 2.6 nM as measured by displacement of [3H]phorbol 12,13-dibutyrate binding in rat cortex synaptosomal membranes). It binds to the C1a and C1b domains of PKC inducing membrane translocation of PKC. Phorbol 12-myristate 13-acetate activates the PKC isoforms PKCα, -β, -γ, -δ, -ε, -η, and -θ but not PKCζ or -ι/λ. Phorbol 12-myristate 13-acetate is a tumor promoter. Phorbol 12-myristate 13-acetate can also induce differentiation of THP-1 monocytes to a macrophage phenotype in vitro.

Product Specifications for Phorbol 12-myristate 13-acetate

Molecular Weight

616.83

Formula

C36H56O8

Storage

Store at -20°C

Purity

≥97% (HPLC)

Chemical Name

(1aR,1bS,4aR,7aS,7bS,8R,9R,9aS)-9a-(Acetyloxy)-1a,1b,4,4a,5,7a,7b,8,9,9a-decahydro-4a,7b-dihydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-5-oxo-1H-cyclopropa[3,4]benz[1,2-e]azulen-9-yl tetradecanoate

CAS Number

16561-29-8

PubChem ID

27924

InChI Key

PHEDXBVPIONUQT-RGYGYFBISA-N

SMILES

OCC1=C[C@]([C@@](C(C)4C)([H])[C@@]4(OC(C)=O)[C@H](OC(CCCCCCCCCCCCC)=O)[C@H]3C)([H])[C@@]3(O)[C@@](C=C(C)C2=O)([H])[C@]2(O)C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 61.68 100

Preparing Stock Solutions for Phorbol 12-myristate 13-acetate

The following data is based on the product molecular weight 616.83.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.62 mL 8.11 mL 16.21 mL
5 mM 0.32 mL 1.62 mL 3.24 mL
10 mM 0.16 mL 0.81 mL 1.62 mL
50 mM 0.03 mL 0.16 mL 0.32 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 202 publications citing the usage of this product.

Product Documents for Phorbol 12-myristate 13-acetate

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Phorbol 12-myristate 13-acetate

For research use only

Related Research Areas

Diabetes
Intracellular Kinases

Citations for Phorbol 12-myristate 13-acetate

Customer Reviews for Phorbol 12-myristate 13-acetate (3)

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  • Action of phorbol 12-myristate 13-acetate in neutrophils
    Name: Sergiy Sylantyev
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Blood
    Verified Customer | Posted 07/13/2023
    Phorbol 12-myristate 13-acetate (PMA) was used to quantify the input of PKC into Ca2+ concentration fluctuations in human neutrophils. Figure: PMA effect on the Ca2+ - induced fluorescence in a single neutrophil (right column).
    Phorbol 12-myristate 13-acetate 1201
  • human isolate PBMCs
    Name: Tahmineh Safaie
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: PBMC
    Verified Customer | Posted 08/04/2020
    human isolate PBMCs were stimulate with 50 ng/ml of PMA for 24 hours and IFN-g secretion was measured with ELISA
    Phorbol 12-myristate 13-acetate 1201
  • PMA stimulation of lymphocytes
    Name: Anonymous
    Assay Type: Ex Vivo
    Cell Line/Tissue: mesenteric lymph node
    Verified Customer | Posted 01/25/2018
    50 ng/ml PMA used to stimulate cells isolated from mesenteric lymphnodes, followed by staining for IL-17A
    Phorbol 12-myristate 13-acetate 1201

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