Home / Elk-1 / Phospho-Elk‑1 (S383) Antibody

Phospho-Elk‑1 (S383) Antibody

Catalog #: PPS021 Datasheet / COA / SDS

Discontinued Product

PPS021 has been discontinued.
View all Elk-1 products.
Detection of Phospho-Elk-1 (S383) by Western Blot
1 Image
Product Details
FAQs
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Phospho-Elk‑1 (S383) Antibody Summary

Species Reactivity
Human, Mouse, Rat, Canine, Primate, Zebrafish
Specificity
Human, mouse and rat ~46 kDa Elk-1 protein phosphorylated at S383
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-S383 of Elk-1
Formulation
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Phospho-Elk-1 (S383) antibody by Western Blot View Larger

Detection of Phospho-Elk-1 (S383) by Western Blot Western blot of phosphorylated and unphosphorylated recombinant Elk-1 protein showing phospho-specific immunolabeling of the ~46 kDa Elk-1 protein.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: Elk-1

Elk-1 (ETS-like protein 1) is a 45 kDa member of the ETS family of serum response element-binding transcription factors. It is 428 amino acids (aa) in length and contains an N-terminal ETS DNA-binding domain (aa 3 - 86), a serum response factor (SRF) binding segment (B box) (aa 148 - 168), a MAPK docking site (D domain) (aa 310 - 334), and a C-terminal transactivation domain (C domain) (aa 336 - 428). Elk-1 is normally SUMOlyated on R230, R249 and R254. This interferes with nuclear localization and activation. It is also bound to p300 in an inactive complex. Phosphorylation by MAPK1 at S383 and S389 removes SUMO protein, allowing for nuclear localization, and activation of the pre-existing p300/Elk-1 complex that is capable of rapidly initiating gene transcription.

References
  1. Yang, S-H. et al. (2003) Mol. Cell 12:63.
  2. Rao, V.N. et al. (1989) Science 244:66.
  3. Li, Q-J. et al. (2003) EMBO J. 22:281.
  4. Salinas, S. et al. (2004) J. Cell Biol. 165:767.
Entrez Gene IDs
2002 (Human); 13712 (Mouse); 314436 (Rat)
Alternate Names
Elk1; Elk-1; ELK1, member of ETS oncogene family; ETS domain-containing protein Elk-1; ETS-like gene 1

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Phospho-Elk‑1 (S383) Antibody

There are currently no reviews for this product. Be the first to review Phospho-Elk‑1 (S383) Antibody and earn rewards!

Have you used Phospho-Elk‑1 (S383) Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review