Detection of Phospho-Elk-1 (S383) by Western Blot
Western blot of phosphorylated and unphosphorylated recombinant Elk-1 protein showing phospho-specific immunolabeling of the ~46 kDa Elk-1 protein.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Elk-1 (ETS-like protein 1) is a 45 kDa member of the ETS family of serum response element-binding transcription factors. It is 428 amino acids (aa) in length and contains an N-terminal ETS DNA-binding domain (aa 3 - 86), a serum response factor (SRF) binding segment (B box) (aa 148 - 168), a MAPK docking site (D domain) (aa 310 - 334), and a C-terminal transactivation domain (C domain) (aa 336 - 428). Elk-1 is normally SUMOlyated on R230, R249 and R254. This interferes with nuclear localization and activation. It is also bound to p300 in an inactive complex. Phosphorylation by MAPK1 at S383 and S389 removes SUMO protein, allowing for nuclear localization, and activation of the pre-existing p300/Elk-1 complex that is capable of rapidly initiating gene transcription.
Yang, S-H. et al. (2003) Mol. Cell 12:63.
Rao, V.N. et al. (1989) Science 244:66.
Li, Q-J. et al. (2003) EMBO J. 22:281.
Salinas, S. et al. (2004) J. Cell Biol. 165:767.
Entrez Gene IDs:
2002 (Human); 13712 (Mouse); 314436 (Rat)
Elk1; Elk-1; ELK1, member of ETS oncogene family; ETS domain-containing protein Elk-1; ETS-like gene 1
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