Phospho-Elk‑1 (S383) Antibody

R&D Systems | Catalog # PPS021

R&D Systems
Discontinued Product
PPS021 has been discontinued. View all Elk-1 products.

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Primate, Zebrafish

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Elk-1 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-S383 of Elk-1

Specificity

Human, mouse and rat ~46 kDa Elk-1 protein phosphorylated at S383

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Phospho-Elk‑1 (S383) Antibody

Detection of Phospho-Elk-1 (S383) antibody by Western Blot

Detection of Phospho-Elk-1 (S383) by Western Blot

Western blot of phosphorylated and unphosphorylated recombinant Elk-1 protein showing phospho-specific immunolabeling of the ~46 kDa Elk-1 protein.

Applications for Phospho-Elk‑1 (S383) Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution
Sample: Phosphorylated recombinant Elk-1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: Elk-1

Elk-1 (ETS-like protein 1) is a 45 kDa member of the ETS family of serum response element-binding transcription factors. It is 428 amino acids (aa) in length and contains an N-terminal ETS DNA-binding domain (aa 3 - 86), a serum response factor (SRF) binding segment (B box) (aa 148 - 168), a MAPK docking site (D domain) (aa 310 - 334), and a C-terminal transactivation domain (C domain) (aa 336 - 428). Elk-1 is normally SUMOlyated on R230, R249 and R254. This interferes with nuclear localization and activation. It is also bound to p300 in an inactive complex. Phosphorylation by MAPK1 at S383 and S389 removes SUMO protein, allowing for nuclear localization, and activation of the pre-existing p300/Elk-1 complex that is capable of rapidly initiating gene transcription.

References

  1. Yang, S-H. et al. (2003) Mol. Cell 12:63.
  2. Rao, V.N. et al. (1989) Science 244:66.
  3. Li, Q-J. et al. (2003) EMBO J. 22:281.
  4. Salinas, S. et al. (2004) J. Cell Biol. 165:767.

Alternate Names

Elk1

Entrez Gene IDs

2002 (Human); 13712 (Mouse); 314436 (Rat)

Gene Symbol

ELK1

Additional Elk-1 Products

Product Documents for Phospho-Elk‑1 (S383) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Phospho-Elk‑1 (S383) Antibody

For research use only

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FAQs

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Associated Pathways

Adipokines & Insulin Signaling Pathways Adipokines & Insulin Signaling Pathway Thumbnail