Phospho-Elk‑1 (S383) Antibody

  
  • Species Reactivity
    Human, Mouse, Rat, Canine, Primate, Zebrafish
  • Specificity
    Human, mouse and rat ~46 kDa Elk-1 protein phosphorylated at S383
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Phosphopeptide corresponding to amino acid residues surrounding the phospho-S383 of Elk-1
  • Formulation
    100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Phospho-Elk-1 (S383) by Western Blot Western blot of phosphorylated and unphosphorylated recombinant Elk-1 protein showing phospho-specific immunolabeling of the ~46 kDa Elk-1 protein.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: Elk-1
Elk-1 (ETS-like protein 1) is a 45 kDa member of the ETS family of serum response element-binding transcription factors. It is 428 amino acids (aa) in length and contains an N-terminal ETS DNA-binding domain (aa 3 - 86), a serum response factor (SRF) binding segment (B box) (aa 148 - 168), a MAPK docking site (D domain) (aa 310 - 334), and a C-terminal transactivation domain (C domain) (aa 336 - 428). Elk-1 is normally SUMOlyated on R230, R249 and R254. This interferes with nuclear localization and activation. It is also bound to p300 in an inactive complex. Phosphorylation by MAPK1 at S383 and S389 removes SUMO protein, allowing for nuclear localization, and activation of the pre-existing p300/Elk-1 complex that is capable of rapidly initiating gene transcription.
  • References:
    1. Yang, S-H. et al. (2003) Mol. Cell 12:63.
    2. Rao, V.N. et al. (1989) Science 244:66.
    3. Li, Q-J. et al. (2003) EMBO J. 22:281.
    4. Salinas, S. et al. (2004) J. Cell Biol. 165:767.
  • Entrez Gene IDs:
    2002 (Human); 13712 (Mouse); 314436 (Rat)
  • Alternate Names:
    Elk1; Elk-1; ELK1, member of ETS oncogene family; ETS domain-containing protein Elk-1; ETS-like gene 1
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