Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) DuoSet IC ELISA

Newer Version Available: DYC1018B-2
NEW

Discontinued Product

DYC1018-2 has been discontinued and is replaced by DYC1018B-2.

Product Details
Citations (28)
FAQs
Reviews (1)

Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) DuoSet IC ELISA Summary

Assay Range
312.0 - 20,000 pg/mL
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: ERK1/ERK2

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44 and 42 kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2.

ERK5, also known as Big Mitogen-activated Protein Kinase 1 (BMK1) and MAPK7, is activated by several mechanisms, including receptor tyrosine kinases, G protein-coupled receptors, and osmotic stress. Like ERK1 and ERK2, ERK5 contains the conserved Thr-Glu-Tyr activation motif in its activation loop. Unlike these ERKs, however, ERK5 contains a unique C-terminal domain that regulates its activation and nuclear translocation.

Long Name:
Extracellular Signal-regulated Kinase 1/2
Alternate Names:
ERK1/ERK2

Citations for Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Decreased concentrations of intracellular signaling proteins in colon cancer patients with BRAF mutations
    Authors: DA Olsen, CEB Thomsen, RF Andersen, JS Madsen, A Jakobsen, I Brandslund
    Sci Rep, 2020;10(1):20113.
    Species: Human
    Sample Types: Tissue Homogenates
  2. Generation of KS-58 as the first K-Ras(G12D)-inhibitory peptide presenting anti-cancer activity in vivo
    Authors: K Sakamoto, T Masutani, T Hirokawa
    Scientific Reports, 2020;10(1):21671.
    Species: Human
    Sample Types: Serum
  3. Neutrophil Immunomodulatory Activity of Natural Organosulfur Compounds
    Authors: IA Schepetkin, LN Kirpotina, AI Khlebnikov, N Balasubram, MT Quinn
    Molecules, 2019;24(9):.
    Species: Human
    Sample Types: Cell Lysates
  4. Inhibition of T Cell Receptor Activation by Semi-Synthetic Sesquiterpene Lactone Derivatives and Molecular Modeling of Their Interaction with Glutathione and Tyrosine Kinase ZAP-70
    Authors: AI Khlebnikov, IA Schepetkin, AS Kishkentae, ZR Shaimerden, GA Atazhanova, SM Adekenov, LN Kirpotina, MT Quinn
    Molecules, 2019;24(2):.
    Species: Human
    Sample Types: Cell Lysates
  5. Increased GPNMB, phospho-ERK1/2, and MMP-9 in cystic fibrosis in association with reduced arylsulfatase B
    Authors: S Bhattachar, L Feferman, G Sharma, JK Tobacman
    Mol. Genet. Metab., 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  6. Evidence that Graves' Ophthalmopathy Immunoglobulins Do Not Directly Activate IGF-1 Receptors
    Authors: B Marcus-Sam, CC Krieger, A Boutin, G Kahaly, S Neumann, MC Gershengor
    Thyroid, 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  7. TRAIL signaling is pro-inflammatory and pro-viral in a murine model of rhinovirus 1B infection
    Authors: Joerg Mattes
    Am. J. Physiol. Lung Cell Mol. Physiol., 2016;0(0):ajplung.00200.
    Species: Mouse
    Sample Types: Tissue Homogenates
  8. Natural nitration of CXCL12 reduces its signaling capacity and chemotactic activity in vitro and abrogates intra-articular lymphocyte recruitment in vivo
    Authors: R Janssens, A Mortier, D Boff, V Vanheule, M Gouwy, C Franck, O Larsen, MM Rosenkilde, J Van Damme, FA Amaral, MM Teixeira, S Struyf, P Proost
    Oncotarget, 2016;7(38):62439-62459.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. Combining nonclinical experiments with translational PKPD modeling to differentiate erlotinib and gefitinib
    Mol Cancer Ther, 2016;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  10. Anti-vascular inflammatory effects of pentacyclic triterpenoids from Astilbe rivularis in�vitro and in�vivo
    Authors: Jong-Sup Bae
    Chem. Biol. Interact., 2016;261(0):127-138.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Microbial sensing by goblet cells controls immune surveillance of luminal antigens in the colon.
    Authors: Knoop, K A, McDonald, K G, McCrate, S, McDole, J R, Newberry, R D
    Mucosal Immunol, 2015;8(1):198-210.
    Species: Human
    Sample Types: Cell Lysates
  12. Sonic Hedgehog activation is implicated in diosgenin-induced megakaryocytic differentiation of human erythroleukemia cells.
    Authors: Ghezali L, Liagre B, Limami Y, Beneytout J, Leger D
    PLoS ONE, 2014;9(4):e95016.
    Species: Human
    Sample Types: Cell Lysates
  13. Cyclopamine and jervine induce COX-2 overexpression in human erythroleukemia cells but only cyclopamine has a pro-apoptotic effect.
    Authors: Ghezali L, Leger D, Limami Y, Cook-Moreau J, Beneytout J, Liagre B
    Exp Cell Res, 2013;319(7):1043-53.
    Species: Human
    Sample Types: Cell Lysates
  14. Activation of brain endothelial cells by interleukin-1 is regulated by the extracellular matrix after acute brain injury.
    Authors: Summers L, Kangwantas K, Rodriguez-Grande B, Denes A, Penny J, Kielty C, Pinteaux E
    Mol Cell Neurosci, 2013;57(0):93-103.
    Species: Rat
    Sample Types: Cell Lysates
  15. Ras oncoproteins transfer from melanoma cells to T cells and modulate their effector functions.
    Authors: Vernitsky H, Rechavi O, Rainy N, Besser M, Nagar M, Schachter J, Lerenthal Y, Ehrlich M, Kloog Y, Goldstein I
    J Immunol, 2012;189(9):4361-70.
    Species: Human
    Sample Types: Cell Lysates
  16. CXCR4 and CCR5 ligands cooperate in monocyte and lymphocyte migration and in inhibition of dual-tropic (R5/X4) HIV-1 infection.
    Authors: Gouwy M, Struyf S, Berghmans N, Vanormelingen C, Schols D, Van Damme J
    Eur. J. Immunol., 2011;41(4):963-73.
    Species: Human
    Sample Types: Cell Lysates
  17. Protein-disulfide isomerase-associated 3 (Pdia3) mediates the membrane response to 1,25-dihydroxyvitamin D3 in osteoblasts.
    Authors: Chen J, Olivares-Navarrete R, Wang Y
    J. Biol. Chem., 2010;285(47):37041-50.
    Species: Mouse
    Sample Types: Cell Lysates
  18. Distinctive ERK and p38 signaling in remote and infarcted myocardium during post-MI remodeling in the mouse.
    Authors: Yeh CC, Li H, Malhotra D, Turcato S, Nicholas S, Tu R, Zhu BQ, Cha J, Swigart PM, Myagmar BE, Baker AJ, Simpson PC, Mann MJ
    J. Cell. Biochem., 2010;109(6):1185-91.
    Species: Mouse
    Sample Types: Tissue Homogenates
  19. Soluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment.
    Authors: Lorquet S, Berndt S, Blacher S, Gengoux E, Peulen O, Maquoi E, Noel A, Foidart JM, Munaut C, Pequeux C
    FASEB J., 2010;24(10):3782-95.
    Species: Human
    Sample Types: Cell Lysates
  20. Demonstration of the equivalent pharmacokinetic/pharmacodynamic dosing strategy in a multiple-dose study of gefitinib.
    Authors: Wang S, Zhou Q, Gallo JM
    Mol. Cancer Ther., 2009;8(6):1438-47.
    Species: Human
    Sample Types: Tissue Homogenates
  21. Citrullination of CXCL12 differentially reduces CXCR4 and CXCR7 binding with loss of inflammatory and anti-HIV-1 activity via CXCR4.
    Authors: Struyf S, Noppen S, Loos T, Mortier A, Gouwy M, Verbeke H, Huskens D, Luangsay S, Parmentier M, Geboes K, Schols D, Van Damme J, Proost P
    J. Immunol., 2009;182(1):666-74.
    Species: Human
    Sample Types: Cell Lysates
  22. Citrullination of CXCL10 and CXCL11 by peptidylarginine deiminase: a naturally occurring posttranslational modification of chemokines and new dimension of immunoregulation.
    Authors: Loos T, Mortier A, Gouwy M, Ronsse I, Put W, Lenaerts JP, Van Damme J, Proost P
    Blood, 2008;112(7):2648-56.
    Species: Hamster
    Sample Types: Cell Lysates
  23. Synergy between coproduced CC and CXC chemokines in monocyte chemotaxis through receptor-mediated events.
    Authors: Gouwy M, Struyf S, Noppen S, Schutyser E, Springael JY, Parmentier M, Proost P, Van Damme J
    Mol. Pharmacol., 2008;74(2):485-95.
    Species: Hamster
    Sample Types: Cell Lysates
  24. In vitro and in vivo pharmacological profile of UFP-512, a novel selective delta-opioid receptor agonist; correlations between desensitization and tolerance.
    Authors: Aguila B, Coulbault L, Boulouard M, Leveille F, Davis A, Toth G, Borsodi A, Balboni G, Salvadori S, Jauzac P, Allouche S
    Br. J. Pharmacol., 2007;152(8):1312-24.
    Species: Human
    Sample Types: Cell Lysates
  25. Enhanced IL-1beta-induced IL-8 production in cystic fibrosis lung epithelial cells is dependent of both mitogen-activated protein kinases and NF-kappaB signaling.
    Authors: Muselet-Charlier C, Roque T, Boncoeur E, Chadelat K, Clement A, Jacquot J, Tabary O
    Biochem. Biophys. Res. Commun., 2007;357(2):402-7.
    Species: Human
    Sample Types: Cell Lysates
  26. Intracellular HIV-Tat expression induces IL-10 synthesis by the CREB-1 transcription factor through Ser133 phosphorylation and its regulation by the ERK1/2 MAPK in human monocytic cells.
    Authors: Gee K, Angel JB, Ma W, Mishra S, Gajanayaka N, Parato K, Kumar A
    J. Biol. Chem., 2006;281(42):31647-58.
    Species: Human
    Sample Types: Cell Lysates
  27. Low dose leflunomide activates PI3K/Akt signalling in erythroleukemia cells and reduces apoptosis induced by anticancer agents.
    Authors: Leger DY, Liagre B, Beneytout JL
    Apoptosis, 2006;11(10):1747-60.
    Species: Human
    Sample Types: Cell Lysates
  28. Effects of the active metabolite of leflunomide, A77 1726, on cytokine release and the MAPK signalling pathway in human rheumatoid arthritis synoviocytes.
    Authors: Vergne-Salle</LastName><F P</Initial, Vergne-Salle P, Leger DY, Bertin P, Treves R, Beneytout JL, Liagre B
    Cytokine, 2005;31(5):335-48.
    Species: Human
    Sample Types: Cell Lysates

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs

Reviews for Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) DuoSet IC ELISA

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) DuoSet IC ELISA?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) DuoSet IC ELISA
By Anonymous on 12/01/2020
Application: Sample Tested: Cell Lysates