Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) DuoSet IC ELISA

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Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) DuoSet IC ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
Cell lysates (100 µL)
Assay Range
469.0 - 30,000 pg/mL
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure phosphorylated human, mouse, and rat ERK1 (T202 / Y204) and ERK2 (T185 / Y187) in cell lysates. An immobilized capture antibody specific for ERK1 / ERK2 binds both phosphorylated and unphosphorylated ERK1 / ERK2. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot

Kit Content

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data

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Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ERK1/ERK2

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44 and 42 kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2.

ERK5, also known as Big Mitogen-activated Protein Kinase 1 (BMK1) and MAPK7, is activated by several mechanisms, including receptor tyrosine kinases, G protein-coupled receptors, and osmotic stress. Like ERK1 and ERK2, ERK5 contains the conserved Thr-Glu-Tyr activation motif in its activation loop. Unlike these ERKs, however, ERK5 contains a unique C-terminal domain that regulates its activation and nuclear translocation.

Long Name:
Extracellular Signal-regulated Kinase 1/2
Alternate Names:

Citations for Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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  1. Knocking-Down CD147/EMMPRIN Expression in CT26 Colon Carcinoma Forces the Cells into Cellular and Angiogenic Dormancy That Can Be Reversed by Interactions with Macrophages
    Authors: G Feigelman, E Simanovich, P Brockmeyer, MA Rahat
    Biomedicines, 2023-03-02;11(3):.
    Species: Mouse
    Sample Types: Cell Lysates
  2. The K-Ras(G12D)-inhibitory peptide KS-58 suppresses growth of murine CT26 colorectal cancer cell-derived tumors
    Authors: K Sakamoto, B Lin, K Nunomura, T Izawa, S Nakagawa
    Scientific Reports, 2022-05-17;12(1):8121.
    Species: Mouse
    Sample Types: Cell Lysates
  3. The Chemokine-Based Peptide, CXCL9(74-103), Inhibits Angiogenesis by Blocking Heparan Sulfate Proteoglycan-Mediated Signaling of Multiple Endothelial Growth Factors
    Authors: A De Zutter, H Crijns, N Berghmans, M García-Cab, L Vanbrabant, N Pörtner, V Vanheule, P Verscheure, MM Siddiquei, AM Abu El-Asr, P Carmeliet, P Van Wielen, I De Meester, J Van Damme, P Proost, S Struyf
    Cancers, 2021-10-12;13(20):.
    Species: Human
    Sample Types: Cell Lysates
  4. Microfluidic Single-Cell Proteomics Assay Chip: Lung Cancer Cell Line Case Study
    Authors: Y Jung, M Son, YR Nam, J Choi, JR Heath, S Yang
    Micromachines, 2021-09-23;12(10):.
    Species: Human
    Sample Types: Cell Lysates
  5. Generation of KS-58 as the first K-Ras(G12D)-inhibitory peptide presenting anti-cancer activity in vivo
    Authors: K Sakamoto, T Masutani, T Hirokawa
    Scientific Reports, 2020-12-10;10(1):21671.
    Species: Human
    Sample Types: Serum
  6. Decreased concentrations of intracellular signaling proteins in colon cancer patients with BRAF mutations
    Authors: DA Olsen, CEB Thomsen, RF Andersen, JS Madsen, A Jakobsen, I Brandslund
    Sci Rep, 2020-11-18;10(1):20113.
    Species: Human
    Sample Types: Tissue Homogenates
  7. Neutrophil Immunomodulatory Activity of Natural Organosulfur Compounds
    Authors: IA Schepetkin, LN Kirpotina, AI Khlebnikov, N Balasubram, MT Quinn
    Molecules, 2019-05-10;24(9):.
    Species: Human
    Sample Types: Cell Lysates
  8. Inhibition of T Cell Receptor Activation by Semi-Synthetic Sesquiterpene Lactone Derivatives and Molecular Modeling of Their Interaction with Glutathione and Tyrosine Kinase ZAP-70
    Authors: AI Khlebnikov, IA Schepetkin, AS Kishkentae, ZR Shaimerden, GA Atazhanova, SM Adekenov, LN Kirpotina, MT Quinn
    Molecules, 2019-01-19;24(2):.
    Species: Human
    Sample Types: Cell Lysates
  9. Evidence that Graves' Ophthalmopathy Immunoglobulins Do Not Directly Activate IGF-1 Receptors
    Authors: B Marcus-Sam, CC Krieger, A Boutin, G Kahaly, S Neumann, MC Gershengor
    Thyroid, 2018-04-30;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  10. Increased GPNMB, phospho-ERK1/2, and MMP-9 in cystic fibrosis in association with reduced arylsulfatase B
    Authors: S Bhattachar, L Feferman, G Sharma, JK Tobacman
    Mol. Genet. Metab., 2018-02-20;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  11. Anti-vascular inflammatory effects of pentacyclic triterpenoids from Astilbe rivularis in�vitro and in�vivo
    Authors: Jong-Sup Bae
    Chem. Biol. Interact., 2016-11-22;261(0):127-138.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. TRAIL signaling is pro-inflammatory and pro-viral in a murine model of rhinovirus 1B infection
    Authors: Joerg Mattes
    Am. J. Physiol. Lung Cell Mol. Physiol., 2016-11-11;0(0):ajplung.00200.
    Species: Mouse
    Sample Types: Tissue Homogenates
  13. Natural nitration of CXCL12 reduces its signaling capacity and chemotactic activity in vitro and abrogates intra-articular lymphocyte recruitment in vivo
    Authors: R Janssens, A Mortier, D Boff, V Vanheule, M Gouwy, C Franck, O Larsen, MM Rosenkilde, J Van Damme, FA Amaral, MM Teixeira, S Struyf, P Proost
    Oncotarget, 2016-09-20;7(38):62439-62459.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  14. Combining nonclinical experiments with translational PKPD modeling to differentiate erlotinib and gefitinib
    Mol Cancer Ther, 2016-09-16;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  15. Microbial sensing by goblet cells controls immune surveillance of luminal antigens in the colon.
    Authors: Knoop, K A, McDonald, K G, McCrate, S, McDole, J R, Newberry, R D
    Mucosal Immunol, 2014-07-09;8(1):198-210.
    Species: Human
    Sample Types: Cell Lysates
  16. Sonic Hedgehog activation is implicated in diosgenin-induced megakaryocytic differentiation of human erythroleukemia cells.
    Authors: Ghezali L, Liagre B, Limami Y, Beneytout J, Leger D
    PLoS ONE, 2014-04-16;9(4):e95016.
    Species: Human
    Sample Types: Cell Lysates
  17. Activation of brain endothelial cells by interleukin-1 is regulated by the extracellular matrix after acute brain injury.
    Authors: Summers L, Kangwantas K, Rodriguez-Grande B, Denes A, Penny J, Kielty C, Pinteaux E
    Mol Cell Neurosci, 2013-10-24;57(0):93-103.
    Species: Rat
    Sample Types: Cell Lysates
  18. Cyclopamine and jervine induce COX-2 overexpression in human erythroleukemia cells but only cyclopamine has a pro-apoptotic effect.
    Authors: Ghezali L, Leger D, Limami Y, Cook-Moreau J, Beneytout J, Liagre B
    Exp Cell Res, 2013-01-25;319(7):1043-53.
    Species: Human
    Sample Types: Cell Lysates
  19. Ras oncoproteins transfer from melanoma cells to T cells and modulate their effector functions.
    Authors: Vernitsky H, Rechavi O, Rainy N, Besser M, Nagar M, Schachter J, Lerenthal Y, Ehrlich M, Kloog Y, Goldstein I
    J Immunol, 2012-10-01;189(9):4361-70.
    Species: Human
    Sample Types: Cell Lysates
  20. CXCR4 and CCR5 ligands cooperate in monocyte and lymphocyte migration and in inhibition of dual-tropic (R5/X4) HIV-1 infection.
    Authors: Gouwy M, Struyf S, Berghmans N, Vanormelingen C, Schols D, Van Damme J
    Eur. J. Immunol., 2011-03-07;41(4):963-73.
    Species: Human
    Sample Types: Cell Lysates
  21. Protein-disulfide isomerase-associated 3 (Pdia3) mediates the membrane response to 1,25-dihydroxyvitamin D3 in osteoblasts.
    Authors: Chen J, Olivares-Navarrete R, Wang Y
    J. Biol. Chem., 2010-09-15;285(47):37041-50.
    Species: Mouse
    Sample Types: Cell Lysates
  22. Soluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment.
    Authors: Lorquet S, Berndt S, Blacher S, Gengoux E, Peulen O, Maquoi E, Noel A, Foidart JM, Munaut C, Pequeux C
    FASEB J., 2010-05-19;24(10):3782-95.
    Species: Human
    Sample Types: Cell Lysates
  23. Distinctive ERK and p38 signaling in remote and infarcted myocardium during post-MI remodeling in the mouse.
    Authors: Yeh CC, Li H, Malhotra D, Turcato S, Nicholas S, Tu R, Zhu BQ, Cha J, Swigart PM, Myagmar BE, Baker AJ, Simpson PC, Mann MJ
    J. Cell. Biochem., 2010-04-15;109(6):1185-91.
    Species: Mouse
    Sample Types: Tissue Homogenates
  24. Demonstration of the equivalent pharmacokinetic/pharmacodynamic dosing strategy in a multiple-dose study of gefitinib.
    Authors: Wang S, Zhou Q, Gallo JM
    Mol. Cancer Ther., 2009-06-09;8(6):1438-47.
    Species: Human
    Sample Types: Tissue Homogenates
  25. Citrullination of CXCL12 differentially reduces CXCR4 and CXCR7 binding with loss of inflammatory and anti-HIV-1 activity via CXCR4.
    Authors: Struyf S, Noppen S, Loos T, Mortier A, Gouwy M, Verbeke H, Huskens D, Luangsay S, Parmentier M, Geboes K, Schols D, Van Damme J, Proost P
    J. Immunol., 2009-01-01;182(1):666-74.
    Species: Human
    Sample Types: Cell Lysates
  26. Citrullination of CXCL10 and CXCL11 by peptidylarginine deiminase: a naturally occurring posttranslational modification of chemokines and new dimension of immunoregulation.
    Authors: Loos T, Mortier A, Gouwy M, Ronsse I, Put W, Lenaerts JP, Van Damme J, Proost P
    Blood, 2008-07-21;112(7):2648-56.
    Species: Hamster
    Sample Types: Cell Lysates
  27. Synergy between coproduced CC and CXC chemokines in monocyte chemotaxis through receptor-mediated events.
    Authors: Gouwy M, Struyf S, Noppen S, Schutyser E, Springael JY, Parmentier M, Proost P, Van Damme J
    Mol. Pharmacol., 2008-05-09;74(2):485-95.
    Species: Hamster, Human
    Sample Types: Cell Lysates
  28. In vitro and in vivo pharmacological profile of UFP-512, a novel selective delta-opioid receptor agonist; correlations between desensitization and tolerance.
    Authors: Aguila B, Coulbault L, Boulouard M, Leveille F, Davis A, Toth G, Borsodi A, Balboni G, Salvadori S, Jauzac P, Allouche S
    Br. J. Pharmacol., 2007-11-05;152(8):1312-24.
    Species: Human
    Sample Types: Cell Lysates
  29. Enhanced IL-1beta-induced IL-8 production in cystic fibrosis lung epithelial cells is dependent of both mitogen-activated protein kinases and NF-kappaB signaling.
    Authors: Muselet-Charlier C, Roque T, Boncoeur E, Chadelat K, Clement A, Jacquot J, Tabary O
    Biochem. Biophys. Res. Commun., 2007-04-02;357(2):402-7.
    Species: Human
    Sample Types: Cell Lysates
  30. Low dose leflunomide activates PI3K/Akt signalling in erythroleukemia cells and reduces apoptosis induced by anticancer agents.
    Authors: Leger DY, Liagre B, Beneytout JL
    Apoptosis, 2006-10-01;11(10):1747-60.
    Species: Human
    Sample Types: Cell Lysates
  31. Intracellular HIV-Tat expression induces IL-10 synthesis by the CREB-1 transcription factor through Ser133 phosphorylation and its regulation by the ERK1/2 MAPK in human monocytic cells.
    Authors: Gee K, Angel JB, Ma W, Mishra S, Gajanayaka N, Parato K, Kumar A
    J. Biol. Chem., 2006-08-18;281(42):31647-58.
    Species: Human
    Sample Types: Cell Lysates
  32. Effects of the active metabolite of leflunomide, A77 1726, on cytokine release and the MAPK signalling pathway in human rheumatoid arthritis synoviocytes.
    Authors: Vergne-Salle</LastName><F P</Initial, Vergne-Salle P, Leger DY, Bertin P, Treves R, Beneytout JL, Liagre B
    Cytokine, 2005-09-07;31(5):335-48.
    Species: Human
    Sample Types: Cell Lysates


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Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) DuoSet IC ELISA
By Jenna Nguyen on 07/09/2019
Sample Tested: 4T1 mouse breast cancer cell line