Phospho-MARCKS (S152/S156) Antibody

(1 citations)   
  • Species Reactivity
    Human, Mouse, Rat, Bovine, Chicken, Xenopus, Zebrafish
  • Specificity
    Specific for the ~83 kDa MARCKS protein phosphorylated at S152/S156 in Western blots of rat brain homogenates.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Phosphopeptide corresponding to amino acid residues surrounding the phospho-S152/S156 of MARCKS
  • Formulation
    100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Phospho-MARCKS (S152/S156) by Western Blot. Western blot of rat brain lysate showing specific immunolabeling of the ~83 kDa MARCKS protein phosphorylated at S152/S156 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda -PPase) for 30 minutes before being exposed to the Anti-Phospho-MARCKS (S152/S156). The immunolabeling is completely eliminated by treatment with lambda -PPase.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: MARCKS
Rat MARCKS (Myristoylated, Alanine-rich C Kinase protein Substrate) is an 83 kDa, 309 amino acid intracellular protein that belongs to the MARCKS family of molecules. It is an actin-organizing molecule and a key substrate for activated protein kinase C (PKC) and PKC-related kinase. MARCKS is normally sequestered on the inner face of the plasma membrane. Here, it is bound to phosphatidylserine (PS) via electrostatic and hydrophobic interactions. Upon integrin ligation ( alpha 5 beta 1), PKC is activated, and phosphorylates MARCKS at S152 and S156. This breaks the PS interaction, forcing MARCKS into the cytosol. Subsequent cytosolic dephosphorylation of MARCKS induces MARCKS-mediated polymerization of actin and its return to the membrane.
  • Long Name:
    Myristoylated Alanine-rich Protein Kinase C Substrate
  • Entrez Gene IDs:
    4082 (Human); 17118 (Mouse); 25603 (Rat)
  • Alternate Names:
    80K-L protein; 80K-L; FLJ14368; MACS; MACSmyristoylated alanine-rich C-kinase substrate; MARCKS; MRACKS; myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L); myristoylated alanine-rich protein kinase C substrate; phosphomyristin; PKCSLFLJ90045; PRKCSL; Protein kinase C substrate, 80 kDa protein, light chain
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. The novel protein kinase C epsilon isoform at the adult neuromuscular synapse: location, regulation by synaptic activity-dependent muscle contraction through TrkB signaling and coupling to ACh release.
    Authors: Obis T, Besalduch N, Hurtado E, Nadal L, Santafe M, Garcia N, Tomas M, Priego M, Lanuza M, Tomas J
    Mol Brain, 2015;8(0):8.
    Species: Rat
    Sample Type: Whole Tissue
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 43  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
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Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG Antibody

WB AF008 1
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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