Detection of Phospho-MARCKS (S152/S156) by Western Blot.
Western blot of rat brain lysate showing specific immunolabeling of the ~83 kDa MARCKS protein phosphorylated at S152/S156 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda -PPase) for 30 minutes before being exposed to the Anti-Phospho-MARCKS (S152/S156). The immunolabeling is completely eliminated by treatment with lambda -PPase.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Rat MARCKS (Myristoylated, Alanine-rich C Kinase protein Substrate) is an 83 kDa, 309 amino acid intracellular protein that belongs to the MARCKS family of molecules. It is an actin-organizing molecule and a key substrate for activated protein kinase C (PKC) and PKC-related kinase. MARCKS is normally sequestered on the inner face of the plasma membrane. Here, it is bound to phosphatidylserine (PS) via electrostatic and hydrophobic interactions. Upon integrin ligation ( alpha 5 beta 1), PKC is activated, and phosphorylates MARCKS at S152 and S156. This breaks the PS interaction, forcing MARCKS into the cytosol. Subsequent cytosolic dephosphorylation of MARCKS induces MARCKS-mediated polymerization of actin and its return to the membrane.
Myristoylated Alanine-rich Protein Kinase C Substrate
Entrez Gene IDs:
4082 (Human); 17118 (Mouse); 25603 (Rat)
80K-L protein; 80K-L; FLJ14368; MACS; MACSmyristoylated alanine-rich C-kinase substrate; MARCKS; MRACKS; myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L); myristoylated alanine-rich protein kinase C substrate; phosphomyristin; PKCSLFLJ90045; PRKCSL; Protein kinase C substrate, 80 kDa protein, light chain
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