Phospho-TAO2 (S181) Antibody

Catalog # Availability Size / Price Qty
PPS037
Detection of Phospho-TAO2 (S181) by Western Blot.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Phospho-TAO2 (S181) Antibody Summary

Species Reactivity
Human, Mouse, Rat, Xenopus
Specificity
This antibody is specific for the ~120 kDa TAO2 phosphorylated at S181 in Western blots of rat cortex lysate.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-S181 of TAO2
Formulation
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Phospho-TAO2 (S181) by Western Blot. View Larger

Detection of Phospho-TAO2 (S181) by Western Blot. Western blot of rat cortex lysate showing specific immunolabeling of the ~120 kDa TAO2 phosphorylated at S181 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 units of lambda phosphatase ( lambda PPase) for 30 minutes before being exposed to the anti-Phospho-TAO2 (S181). The immunolabeling is completely eliminated by treatment with lambda PPase.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ ‑20° C is recommended. Product is stable at ≤ ‑20° C for at least 1 year.

Background: TAO2

TAO2 (thousand and one amino acid protein kinase 2; also known as MAPK3) is a 160 kDa, 1235 amino acid (aa) kinase that belongs to the Serine/Threonine protein kinase family. It is part of a stress-sensitive kinase cascade that activates and phosphorylates MEK3, 4, and 6. The molecule is reported to be an intracellular serpentine transmembrane (TM) molecule with four or five TM segments that involve aa 965-1065. The N-terminal region contains a protein kinase domain (aa 28-231) followed by glutamic acid and serine rich regions (aa 330-405). Phosphorylation of S181 activates the enzyme. It is unclear if this is an autophosphorylation event. One isoform exists that utilizes an alternate start site at position 174, followed by a unique 44 aa N-terminus. This lacks the catalytic serine found in the standard isoform. Its function is unclear.

References
  1. Chen, Z. et al. (1999) J. Biol. Chem. 274:28803.
  2. Chen, Z. and M.H. Cobb (2001) J. Biol. Chem. 276:16070.
  3. Moore, T.M. et al. (2000) J. Biol. Chem. 275:4311.
  4. Zhou, T. et al. (2004) Structure 12:1891.
  5. SwissProt Accession #: Q9UL54.
Long Name
Serine/Threonine-protein Kinase TAO2
Entrez Gene IDs
9344 (Human); 381921 (Mouse); 64666 (Rat)
Alternate Names
EC 2.7.11; KFC-C; KIAA0881PSK-1; Kinase from chicken homolog C; MAP3K17; MAP3K17PSK1-BETA; Prostate-derived STE20-like kinase 1; PSK1; PSK1EC 2.7.11.1; PSKhKFC-C; serine/threonine-protein kinase TAO2; TAO kinase 2; TAO1; TAO2; TAO2prostate derived STE20-like kinase PSK; TAOK2; Thousand and one amino acid protein 2; thousand and one amino acid protein kinase

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Phospho-TAO2 (S181) Antibody

There are currently no reviews for this product. Be the first to review Phospho-TAO2 (S181) Antibody and earn rewards!

Have you used Phospho-TAO2 (S181) Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review