Phospholipase A2 XII Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-19808

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (97%), Mouse (97%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Phospholipase A2 XII. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (83%).

Localization

Secreted; Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Phospholipase A2 XII Antibody - BSA Free

Western Blot: Phospholipase A2 XII Antibody [NBP2-19808]

Western Blot: Phospholipase A2 XII Antibody [NBP2-19808]

Western Blot: Phospholipase A2 XII Antibody [NBP2-19808] - Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: Phospholipase A2 XII Antibody [NBP2-19808]

Immunohistochemistry-Paraffin: Phospholipase A2 XII Antibody [NBP2-19808]

Immunohistochemistry-Paraffin: Phospholipase A2 XII Antibody [NBP2-19808] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.

Applications for Phospholipase A2 XII Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Phospholipase A2 XII

Secreted phospholipase A2 (sPLA2) enzymes liberate arachidonic acid from phospholipids for production of eicosanoids and exert a variety of physiologic and pathologic effects. Group XII sPLA2s, such as PLA2G12A, have relatively low specific activity and are structurally and functionally distinct from other sPLA2s (Gelb et al., 2000 [PubMed 11031251]).[supplied by OMIM]

Alternate Names

EC 3.1.1.4, group XII secreted phospholipase A2, group XIIA secreted phospholipase A2, group XIIA secretory phospholipase A2, GXII, GXII sPLA2, Phosphatidylcholine 2-acylhydrolase 12A, phospholipase A2, group XII, phospholipase A2, group XIIA, PLA2G12, ROSSY, sPLA2-XII

Gene Symbol

PLA2G12A

UniProt

Additional Phospholipase A2 XII Products

Product Documents for Phospholipase A2 XII Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Phospholipase A2 XII Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Phospholipase A2 XII Antibody - BSA Free

Showing  1 - 2 of 2 FAQs Showing All
  • Q:

    Our customer asked about the cross reactivity of PLA2G12A antibodies. Do you have any info if these antibodies recognize other phospholipases?

    A: Unless specifically indicated on the datasheet they are not tested to recognize other phospholipases. The immunogen information is present for all of those so that might give some clues to the customer as to whether they might have some reactivity, but as far as direct testing is concerned on the other phospholipases this has not been performed by the lab.

  • Q: Our customer asked about the cross reactivity of PLA2G12A antibodies. Do you have any info if these antibodies?

    A: Unless specifically indicated on the datasheet they are not tested to recognize other phospholipases. The immunogen information is present for all of those so that might give some clues to the customer as to whether they might have some reactivity, but as far as direct testing is concerned on the other phospholipases this has not been performed by the lab.

  • Q:

    Our customer asked about the cross reactivity of PLA2G12A antibodies. Do you have any info if these antibodies recognize other phospholipases?

    A: Unless specifically indicated on the datasheet they are not tested to recognize other phospholipases. The immunogen information is present for all of those so that might give some clues to the customer as to whether they might have some reactivity, but as far as direct testing is concerned on the other phospholipases this has not been performed by the lab.

  • Q: Our customer asked about the cross reactivity of PLA2G12A antibodies. Do you have any info if these antibodies?

    A: Unless specifically indicated on the datasheet they are not tested to recognize other phospholipases. The immunogen information is present for all of those so that might give some clues to the customer as to whether they might have some reactivity, but as far as direct testing is concerned on the other phospholipases this has not been performed by the lab.

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