Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-73394

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1B8

Format

Azide and BSA Free
View all Phosphoribosyl Pyrophosphate Amidotransferase Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 42-278 of human PPAT(NP_002694) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

57.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images

Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8)Azide and BSA Free [NBP2-73394]

Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8)Azide and BSA Free [NBP2-73394]

Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Phosphoribosyl Pyrophosphate Amidotransferase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and
Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394]

Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394]

Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394] - Staining of paraffin-embedded Human Kidney tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.
Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8)Azide and BSA Free [NBP2-73394]

Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8)Azide and BSA Free [NBP2-73394]

Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-Phosphoribosyl Pyrophosphate Amidotransferase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: hum
Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394]

Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394]

Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.
Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394]

Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394]

Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.
Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394]

Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394]

Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.
Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394]

Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394]

Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394] - Staining of paraffin-embedded Human colon tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.
Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394]

Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394]

Immunohistochemistry: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) - Azide and BSA Free [NBP2-73394] - Staining of paraffin-embedded Human liver tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.

Applications

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Phosphoribosyl Pyrophosphate Amidotransferase

Phosphoribosyl Pyrophosphate Amidotransferase is encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. This gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway, are located in close proximity on chromosome 4.

Alternate Names

ATASE, EC 2.4.2.14, glutamine phosphoribosylpyrophosphatate amidotransferase, Glutamine phosphoribosylpyrophosphate amidotransferase, glutamine PRPP amidotransferase, GPATamidophosphoribosyltransferase, phosphoribosyl pyrophosphate amidotransferase, PRAT

Gene Symbol

PPAT

Additional Phosphoribosyl Pyrophosphate Amidotransferase Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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