Phosphoribosyl Pyrophosphate Amidotransferase Antibody

Name: Phosphoribosyl Pyrophosphate Amidotransferase Antibody
Brand: Novus Biologicals
SKU: NBP2-19812
Price: 449 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-19812

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (90%), Mouse (94%), Rat (91%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Phosphoribosyl Pyrophosphate Amidotransferase. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Phosphoribosyl Pyrophosphate Amidotransferase Antibody

Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody [NBP2-19812]

Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody [NBP2-19812] - Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 7.5% SDS PAGE diluted at 1:1000

Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody [NBP2-19812] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PPAT antibody (NBP2-19812) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for Phosphoribosyl Pyrophosphate Amidotransferase Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Phosphoribosyl Pyrophosphate Amidotransferase

The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. This gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway, are located in close proximity on chromosome 4. [provided by RefSeq]

Alternate Names

ATASE, EC 2.4.2.14, glutamine phosphoribosylpyrophosphatate amidotransferase, Glutamine phosphoribosylpyrophosphate amidotransferase, glutamine PRPP amidotransferase, GPATamidophosphoribosyltransferase, phosphoribosyl pyrophosphate amidotransferase, PRAT

Gene Symbol

PPAT

UniProt

Q06203 (Human)

Additional Phosphoribosyl Pyrophosphate Amidotransferase Products

Product Documents for Phosphoribosyl Pyrophosphate Amidotransferase Antibody

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Certificate of Analysis

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Product Specific Notices for Phosphoribosyl Pyrophosphate Amidotransferase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.

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