Piccolo Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-90250

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Piccolo Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PITTLDSITTVYTEPVDMITKFEDSEEISSSTYFPGSIIDYPEEISVSLDRTAPPDGRASADHIVISLSDMASSIIESVVPKPEGPVADTVSTDLLISEKDPVKKAKKETGNGIILEVLEA

Marker

Synaptic Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Piccolo Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Piccolo Antibody [NBP1-90250]

Immunocytochemistry/ Immunofluorescence: Piccolo Antibody [NBP1-90250]

Immunocytochemistry/Immunofluorescence: Piccolo Antibody [NBP1-90250] - Staining of human cell line U-251 MG shows localization to nuclear speckles & plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Piccolo Antibody [NBP1-90250]

Immunohistochemistry-Paraffin: Piccolo Antibody [NBP1-90250]

Immunohistochemistry-Paraffin: Piccolo Antibody [NBP1-90250] - Staining of human cerebral cortex shows strong positivity in neuropil.
Immunohistochemistry-Paraffin: Piccolo Antibody [NBP1-90250]

Immunohistochemistry-Paraffin: Piccolo Antibody [NBP1-90250]

Immunohistochemistry-Paraffin: Piccolo Antibody [NBP1-90250] - Staining of human testis shows weak cytoplasmic positivity in cells in seminiferous ducts.
Piccolo Antibody - BSA Free Immunohistochemistry: Piccolo Antibody - BSA Free [NBP1-90250]

Immunohistochemistry: Piccolo Antibody - BSA Free [NBP1-90250]

Staining of human cerebellum shows strong positivity in neuropil.

Applications for Piccolo Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Piccolo

Piccolo is a presynaptic cytomatrix protein concentrated at the presynaptic side of synaptic junctions. Piccolo is a large protein which constists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains (PBH-domains) and C-terminal PDZ and C2 domains. It is usually found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Recently piccolo has been shown to interfere with clathrin mediated endocytosis by binding to the F-actin and dynamin binding protein Abp1. Piccolo is highly expressed in brain with low levels found in stomach. It is not detected in other tissues analyzed including adrenal gland, testis and pancreas.

Long Name

Presynaptic Cytomatrix Protein

Alternate Names

ACZ, Aczonin, PCLO

Gene Symbol

PCLO

Additional Piccolo Products

Product Documents for Piccolo Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Piccolo Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Piccolo Antibody - BSA Free

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Protocols

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