PIEZO1 Antibody Blocking Peptide
Novus Biologicals | Catalog # NBP1-78446PEP
Product Specifications
Description
Application Notes
Specificity
Protein / Peptide Type
Formulation, Preparation, and Storage
NBP1-78446PEP
| Formulation | Peptide dissolved in dH2O. Contains no BSA. |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -80C. Avoid freeze-thaw cycles. |
Background: PIEZO1
Long Name
Alternate Names
Gene Symbol
Additional PIEZO1 Products
Product Documents for PIEZO1 Antibody Blocking Peptide
Certificate of Analysis
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Product Specific Notices for PIEZO1 Antibody Blocking Peptide
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
Citations for PIEZO1 Antibody Blocking Peptide
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FAQs for PIEZO1 Antibody Blocking Peptide
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Q: I received the Piezo-1 blocking peptide this morning. In the past, I have blocked antibodies for IHC by pre-absorbing the antibody with a 5-fold excess of blocking peptide for 2 hours at 4 degrees C before diluting the blocked mixture and applying to the slides. Can you confirm that this protocol is what you would recommend for effective blocking, or otherwise, suggest appropriate conditions. I am also assuming from the data sheet you sent that you recommend to reconstitute the peptide in water.
A: The peptide you received is not lyophilized but in solution form and the protocol you mentioned seems great.
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Q: We have a customer who purchased NBP1-78446 and NBP1-78446PEP (PO12-01725/PO12-01822). The customer uses the antibody at a dilution of 1:10,000 in IHC successfully, but would like to use the blocking peptide to verify their IHC results. The customer would like to receive the recommended blocking peptide protocol for NBP1-78446PEP - most important, which is the recommended concentration/fold relation to antibody the customer should use!
A: We have a general recommended protocol for blocking peptides, which should be useful for you. You will see that in point 2., we say 'add the blocking peptide solution at a 5-10:1 ratio to your antibody, i.e. if you added 1 ug of antibody, add 5-10 ug of blocking peptide'.
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Q: I received the Piezo-1 blocking peptide this morning. In the past, I have blocked antibodies for IHC by pre-absorbing the antibody with a 5-fold excess of blocking peptide for 2 hours at 4 degrees C before diluting the blocked mixture and applying to the slides. Can you confirm that this protocol is what you would recommend for effective blocking, or otherwise, suggest appropriate conditions. I am also assuming from the data sheet you sent that you recommend to reconstitute the peptide in water.
A: The peptide you received is not lyophilized but in solution form and the protocol you mentioned seems great.
-
Q: We have a customer who purchased NBP1-78446 and NBP1-78446PEP (PO12-01725/PO12-01822). The customer uses the antibody at a dilution of 1:10,000 in IHC successfully, but would like to use the blocking peptide to verify their IHC results. The customer would like to receive the recommended blocking peptide protocol for NBP1-78446PEP - most important, which is the recommended concentration/fold relation to antibody the customer should use!
A: We have a general recommended protocol for blocking peptides, which should be useful for you. You will see that in point 2., we say 'add the blocking peptide solution at a 5-10:1 ratio to your antibody, i.e. if you added 1 ug of antibody, add 5-10 ug of blocking peptide'.