PIK3C2A Antibody (OTI3H2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-73405

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3H2

Format

Azide and BSA Free
View all PIK3C2A Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 230-560 of human PIK3C2A (NP_002636) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

190.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PIK3C2A Antibody (OTI3H2) - Azide and BSA Free

Western Blot: PIK3C2A Antibody (OTI3H2)Azide and BSA Free [NBP2-73405]

Western Blot: PIK3C2A Antibody (OTI3H2)Azide and BSA Free [NBP2-73405]

Western Blot: PIK3C2A Antibody (OTI3H2) - Azide and BSA Free [NBP2-73405] - E.coli lysate (left lane) and E.coli lysate expressing human recombinant protein fragment corresponding to amino acids 230-560 of human PIK3C2A were separated by SDS-PAGE and immunoblotted with PIK3C2A.
Western Blot: PIK3C2A Antibody (OTI3H2)Azide and BSA Free [NBP2-73405]

Western Blot: PIK3C2A Antibody (OTI3H2)Azide and BSA Free [NBP2-73405]

Western Blot: PIK3C2A Antibody (OTI3H2) - Azide and BSA Free [NBP2-73405] - Analysis of HT29 cell lysate (35ug) by using PIK3C2A monoclonal antibody.

Applications for PIK3C2A Antibody (OTI3H2) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PIK3C2A

PIK3C2A belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling.

Long Name

Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha

Alternate Names

EC 2.7.1.137, EC 2.7.1.153, EC 2.7.1.154, PI3K-C2-alpha

Gene Symbol

PIK3C2A

Additional PIK3C2A Products

Product Documents for PIK3C2A Antibody (OTI3H2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PIK3C2A Antibody (OTI3H2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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