Pin1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-28699

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Pin1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human protein (peptidylprolyl cis/trans isomerase) NIMA-interacting 1 using the numbering given in entry NP_006212.1 (GeneID 5300).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Pin1 Antibody - BSA Free

Immunoprecipitation: Pin1 Antibody [NBP1-28699]

Immunoprecipitation: Pin1 Antibody [NBP1-28699]

Immunoprecipitation: Pin1 Antibody [NBP1-28699] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PIN1 antibody NBP1-28699 used for IP at 10 ug/mg lysate. PIN1 was not successfully immunoprecipitated by rabbit anti-PIN1 antibody NBP1-28713, which recognizes a downstream epitope. For blotting immunoprecipitated PIN1, NBP1-28713 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Pin1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

10 ug/mg lysate
Application Notes
It is not recommended for western analysis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Pin1

Peptidyl-prolyl cis-trans isomerase NIMA-interacting protein 1 (Pin1) is an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Pin1 displays a preference for an acidic residue N-terminal to the isomerized proline bond, catalyzing pSer/Thr-Pro cis/trans isomerizations.

Long Name

Protein [Peptidyl-prolyl cis/trans Isomerase] NIMA-interacting 1

Alternate Names

dod

Entrez Gene IDs

5300 (Human)

Gene Symbol

PIN1

UniProt

Q13526

Additional Pin1 Products

Product Documents for Pin1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Pin1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

NFkB Pathway
p53 Pathway

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Protocols

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FAQs

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