PLK2 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-15078
![Western Blot: PLK2 Antibody [NBP2-15078]](https://resources.rndsystems.com/images/products/PLK2-Antibody-Western-Blot-NBP2-15078-img0001.jpg "Western Blot: PLK2 Antibody [NBP2-15078]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (98%), Rat (98%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human PLK2. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (89%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit PLK2 Antibody - BSA Free (NBP2-15078) is a polyclonal antibody validated for use in IHC and WB. Anti-PLK2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PLK2 Antibody - BSA Free
Western Blot: PLK2 Antibody [NBP2-15078]
Western Blot: PLK2 Antibody [NBP2-15078] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE; antibody diluted at 1:1000.![Immunohistochemistry-Paraffin: PLK2 Antibody [NBP2-15078]](https://resources.rndsystems.com/images/products/PLK2-Antibody-Immunohistochemistry-Paraffin-NBP2-15078-img0002.jpg "Immunohistochemistry-Paraffin: PLK2 Antibody [NBP2-15078]")
Immunohistochemistry-Paraffin: PLK2 Antibody [NBP2-15078]
Immunohistochemistry-Paraffin: PLK2 Antibody [NBP2-15078] - Mouse colon, dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: PLK2 Antibody - BSA Free [NBP2-15078] -
HBI-002 effects on autophagy, lysosomes and cathepsin D.a Immunoblots of autophagy and lysosomal activity markers. b Quantification of autophagy and lysosomal activity markers. Ctsd (p = 0.002, 1-beta = 0.99, N = 8, 4 per group) and Plk2 (p = 0.006, 1-beta = 0.99, N = 8, 4 per group), all other markers p > 0.05. c Representative photomicrographs of sir-lysosome staining in SH-SY5Y human neuroblastomas. d Quantification of sir-lysosome staining p = 0.0002, 1-beta = 0.95, N = 38 cells, air treated n = 23, CO-treated n = 14. Error bars represent the mean +/- SEM. Data are statistically different from each other with *** P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41531-024-00763-6), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for PLK2 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PLK2
Long Name
Serine/threonine-protein kinase PLK2
Alternate Names
EC 2.7.11.21, hPlk2, hSNK, PLK-2, Polo-like kinase 2, SNK
Gene Symbol
PLK2
UniProt
Additional PLK2 Products
Product Documents for PLK2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PLK2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Citations for PLK2 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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