PMEL17/SILV Antibody (JE42-47)

Western Blot: PMEL17/SILV Antibody (JE42-47) [NBP3-32586]

Western Blot: PMEL17/SILV Antibody (JE42-47) [NBP3-32586] - Western blot analysis of PMEL17/SILV on SK-MEL-28 cell lysates with Rabbit anti-PMEL17/SILV antibody (NBP3-32586) at 1/1,000 dilution.

Lysates/proteins at 20 ug/Lane.

Predicted band size: 70 kDa
Observed band size: 100 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32586) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: PMEL17/SILV Antibody (JE42-47) [NBP3-32586]

Immunohistochemistry: PMEL17/SILV Antibody (JE42-47) [NBP3-32586] - Immunohistochemical analysis of paraffin-embedded human melanoma tissue with Rabbit anti-PMEL17/SILV antibody (NBP3-32586) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32586) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: PMEL17/SILV Antibody (JE42-47) [NBP3-32586]

Western blot analysis of SK-MEL-28 cell lysates with NBP3-32586 at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 70 kDa Observed band size: 100 kDa Exposure time: 2 minutes;