Q: What is the procedure to denature podoplanin protein?

A: We denature our proteins by diluting them with loading buffer and boiling them at 95 degrees Celsius for 5-10 minutes. If reducing conditions are desired, we add 5% BME or DTT to the loading buffer prior to boiling the sample. With larger proteins (+200kDa) we will heat the samples at 65 degrees Celsius for 10 minutes.

Recombinant Human Podoplanin His Protein

Novus Biologicals | Catalog # NBC1-18511

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Product Documents

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all Podoplanin Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 99-207 of Human Podoplanin

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MASTGQPEDD TETTGLEGGV AMPGAEDDVV TPGTSEDRYK SGLTTLVATS VNSVTGIRIE DLPTSESTVH AQEQSPSATA SNVATSHSTE KVDGDTQTTV EKDGLSTVTL

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

13.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Podoplanin His Protein

SDS-PAGE: Recombinant Human Podoplanin His Protein [NBC1-18511]

SDS-Page: Recombinant Human Podoplanin Protein [NBC1-18511] - PDPN, 13.4 kDa (130 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Formulation, Preparation, and Storage

NBC1-18511

Formulation	20 mM Tris-HCl buffer (pH 7.5), 0.1 M NaCl
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Podoplanin

Podoplanin(PDPN) is a small mucin-like transmembrane protein, widely expressed in various specialized cell types throughout the body. This is a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. Recombinant human PDPN protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.

Long Name

Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A

Alternate Names

Aggrus, Gp38, PDPN, RANDAM-2, T1A-2

Gene Symbol

PDPN

Additional Podoplanin Products

Product Documents for Recombinant Human Podoplanin His Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human Podoplanin His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

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Endothelial Cell Markers

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