POFUT2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-93894

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all POFUT2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 240-370 of human POFUT2 (NP_056042.1). MVFARHLREVGDEFRSRHLNSTDDADRIPFQEDWMKMKVKLGSALGGPYLGVHLRRKDFIWGHRQDVPSLEGAVRKIRSLMKTHRLDKVFVATDAVRKEYEELKKLLPEMVRFEPTWEELELYKDGGVAII

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POFUT2 Antibody - Azide and BSA Free

Western Blot: POFUT2 AntibodyAzide and BSA Free [NBP2-93894]

Western Blot: POFUT2 AntibodyAzide and BSA Free [NBP2-93894]

Western Blot: POFUT2 Antibody [NBP2-93894] - Analysis of extracts of various cell lines, using POFUT2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunocytochemistry/ Immunofluorescence: POFUT2 Antibody - Azide and BSA Free [NBP2-93894]

Immunocytochemistry/ Immunofluorescence: POFUT2 Antibody - Azide and BSA Free [NBP2-93894]

Immunocytochemistry/Immunofluorescence: POFUT2 Antibody [NBP2-93894] - Immunofluorescence analysis of U2OS cells using POFUT2 Rabbit pAb (NBP2-93894) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: POFUT2 Antibody - Azide and BSA Free [NBP2-93894]

Immunocytochemistry/ Immunofluorescence: POFUT2 Antibody - Azide and BSA Free [NBP2-93894]

Immunocytochemistry/Immunofluorescence: POFUT2 Antibody [NBP2-93894] - Immunofluorescence analysis of H9C2 cells using POFUT2 Rabbit pAb (NBP2-93894) at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for POFUT2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: POFUT2

Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).[supplied by OMIM]

Alternate Names

C21orf80, chromosome 21 open reading frame 80, FUT13EC 2.4.1.221, GDP-fucose protein O-fucosyltransferase 2, KIAA0958, O-FucT-2, Peptide-O-fucosyltransferase 2, protein O-fucosyltransferase 2

Gene Symbol

POFUT2

Additional POFUT2 Products

Product Documents for POFUT2 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for POFUT2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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