POLR1A Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-30153

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all POLR1A Primary Antibodies »

Product Specifications

Immunogen

Between 1350 and 1400

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POLR1A Antibody - BSA Free

POLR1A Antibody

Immunoprecipitation:POLR1A AntibodyNBP3-30153] -

Immunoprecipitation:POLR1A AntibodyNBP3-30153] -Detection of human POLR1A/RPA194 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-POLR1A/RPA194 antibody NBP3-30153 (lot NBP3-30153-1) used for IP at 6 µg per reaction. POLR1A/RPA194 was also immunoprecipitated by rabbit anti-POLR1A/RPA194.. For blotting immunoprecipitated POLR1A/RPA194, was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for POLR1A Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: POLR1A

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition

Long Name

DNA-directed RNA polymerase I subunit RPA1

Alternate Names

A190, RPA194

Gene Symbol

POLR1A

Additional POLR1A Products

Product Documents for POLR1A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for POLR1A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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