Ponceau S Staining Solution

Tocris Bioscience | Catalog # 5225

Stain used to detect protein bands after Western blotting
Tocris Bioscience

Key Product Details

Description

Stain used to detect protein bands after Western blotting
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Product Description

Ponceau S Staining Solution is a stain used to detect protein bands after Western blotting.

Product Specifications for Ponceau S Staining Solution

Molecular Weight

760.57

Formula

C22H12N4Na4O13S4

Storage

Store at RT

Chemical Name

3-Hydroxy-4-[2-[2-sulfo-4-[2-(4-sulfophenyl)diazenyl]phenyl]diazenyl]-2,7-naphthalenedisulfonic acid tetrasodium salt

CAS Number

6226-79-5

PubChem ID

101933731

InChI Key

KQHKSGRIBYJYFX-UHFFFAOYSA-J

SMILES

OC(C(S(=O)(O[Na])=O)=CC4=C3C=CC(S(=O)(O[Na])=O)=C4)=C3/N=N/C(C=C2)=C(S(=O)(O[Na])=O)C=C2/N=N/C1=CC=C(S(=O)(O[Na])=O)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solubility Soluble in 5% acetic acid/water (supplied as a 0.1% w/v solution)

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 6 publications citing the usage of this product.

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Product Documents for Ponceau S Staining Solution

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Ponceau S Staining Solution

For research use only

Citations for Ponceau S Staining Solution

Customer Reviews for Ponceau S Staining Solution (4)

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  • Ponceau S Staining Solution
    Name: Chi Ngo
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HEK293
    Verified Customer | Posted 09/06/2025
    I used Ponceau S Staining Solution to ensure equal loadings and optimal transfer (from gels to membrane) in western blotting.
    Ponceau S Staining Solution 5225
  • Stain to detect protein bands and confirm sucessful transfer prior to antibody incubation.
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HEK293
    Verified Customer | Posted 12/23/2019
    The stain on the membrane can be washed off with PBS or TBS so that it can be further incubated with the primary antibody for immunodetection.
    Protein samples were resolved in NuPAGE 4–12% Bis-Tris gel (Invitrogen) and transferred to 0.2 µm PVDF membrane. The membrane was stained with Ponceau S solution for less than a minute to detect protein bands and confirm successful sample run and transfer (lane 2-6) or not (lane 1).
    Ponceau S Staining Solution 5225
  • Best staining for tracking protein transfer in western blot
    Name: Luiz Alexandre Magno
    Species: Mouse
    Assay Type: In Vitro
    Verified Customer | Posted 07/03/2018
    1. To get a clear background, wash the membrane quickly in PBS after staining 2. Ponceau red stain is not stable and will disappear if you destain longer. 3. It is advisable to mark with a pencil the position of the bands of interest if you want to cut a membrane in various sections
    We use Ponceau S (Cat. No. 5225) to confirm successful transfer of proteins from the gel onto nitrocellulose membranes during the transfer step of western blot analysis. This reagent stains protein bands very quickly (less than 1 min) and has the big advantage to be easily destained after a quick wash step that can be made with water or PBS. It is also used in our lab to detect protein degradation or any other abnormalities like overloading (as shown in the figure, the line #9 contains a protein sample degrade). Moreover, it is also useful to select the membrane area to be cut after transfer, based on the migration pattern of the protein standards (line #1). This enables one to incubate the different membrane sections with various primary antibodies.
    Ponceau S Staining Solution 5225
  • Western blot confirmation
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Verified Customer | Posted 01/05/2018
    Western blot confirmation

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