Ponceau S Staining Solution

Catalog # Availability Size / Price Qty
Ponceau S Staining Solution | CAS No. 6226-79-5 | Buffers, Chelators and Reagents
1 Image
Description: Stain used to detect protein bands after Western blotting

Chemical Name: 3-Hydroxy-4-[2-[2-sulfo-4-[2-(4-sulfophenyl)diazenyl]phenyl]diazenyl]-2,7-naphthalenedisulfonic acid tetrasodium salt

Product Details
Reviews (3)

Biological Activity

Stain used to detect protein bands after Western blotting.

Technical Data

Soluble in 5% acetic acid/water (supplied as a 0.1% w/v solution)
Store at RT

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets

or select another batch:
Reconstitution Calculator
Molarity Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Molarity Calculator


*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).


No product specific FAQs exist for this product, however you may

View all Small Molecule FAQs

Reviews for Ponceau S Staining Solution

Average Rating: 4.3 (Based on 3 Reviews)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Ponceau S Staining Solution?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Stain to detect protein bands and confirm sucessful transfer prior to antibody incubation.
By Anonymous on 12/23/2019
Application: Species: Human

Protein samples were resolved in NuPAGE 4–12% Bis-Tris gel (Invitrogen) and transferred to 0.2 µm PVDF membrane. The membrane was stained with Ponceau S solution for less than a minute to detect protein bands and confirm successful sample run and transfer (lane 2-6) or not (lane 1).

Best staining for tracking protein transfer in western blot
By Luiz Alexandre Magno on 07/03/2018
Application: Species: Mouse

We use Ponceau S (Cat. No. 5225) to confirm successful transfer of proteins from the gel onto nitrocellulose membranes during the transfer step of western blot analysis. This reagent stains protein bands very quickly (less than 1 min) and has the big advantage to be easily destained after a quick wash step that can be made with water or PBS. It is also used in our lab to detect protein degradation or any other abnormalities like overloading (as shown in the figure, the line #9 contains a protein sample degrade). Moreover, it is also useful to select the membrane area to be cut after transfer, based on the migration pattern of the protein standards (line #1). This enables one to incubate the different membrane sections with various primary antibodies.

PMID: 26585834

Western blot confirmation
By Anonymous on 01/05/2018
Application: Species: Human

Western blot confirmation

Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris is a Bio-Techne brand.