Ponceau S Staining Solution

Catalog # Availability Size / Price Qty
5225/500ML
Ponceau S Staining Solution | CAS No. 6226-79-5 | Buffers, Chelators and Reagents
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Description: Stain used to detect protein bands after Western blotting

Chemical Name: 3-Hydroxy-4-[2-[2-sulfo-4-[2-(4-sulfophenyl)diazenyl]phenyl]diazenyl]-2,7-naphthalenedisulfonic acid tetrasodium salt

Product Details
Reviews (3)

Biological Activity

Stain used to detect protein bands after Western blotting.

Technical Data

M.Wt:
760.57
Formula:
C22H12N4Na4O13S4
Solubility:
Soluble in 5% acetic acid/water (supplied as a 0.1% w/v solution)
Storage:
Store at RT
CAS No:
6226-79-5

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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Reviews for Ponceau S Staining Solution

Average Rating: 4.3 (Based on 3 Reviews)

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Stain to detect protein bands and confirm sucessful transfer prior to antibody incubation.
By Anonymous on 12/23/2019
Application: Species: Human

Protein samples were resolved in NuPAGE 4–12% Bis-Tris gel (Invitrogen) and transferred to 0.2 µm PVDF membrane. The membrane was stained with Ponceau S solution for less than a minute to detect protein bands and confirm successful sample run and transfer (lane 2-6) or not (lane 1).


Best staining for tracking protein transfer in western blot
By Luiz Alexandre Magno on 07/03/2018
Application: Species: Mouse

We use Ponceau S (Cat. No. 5225) to confirm successful transfer of proteins from the gel onto nitrocellulose membranes during the transfer step of western blot analysis. This reagent stains protein bands very quickly (less than 1 min) and has the big advantage to be easily destained after a quick wash step that can be made with water or PBS. It is also used in our lab to detect protein degradation or any other abnormalities like overloading (as shown in the figure, the line #9 contains a protein sample degrade). Moreover, it is also useful to select the membrane area to be cut after transfer, based on the migration pattern of the protein standards (line #1). This enables one to incubate the different membrane sections with various primary antibodies.

PMID: 26585834

Western blot confirmation
By Anonymous on 01/05/2018
Application: Species: Human

Western blot confirmation


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