Porcine IgG, Fab Native Protein

Name: Porcine IgG, Fab Native Protein
Brand: Novus Biologicals
SKU: NBP1-97010
Price: 299 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-97010

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

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Product Specifications

Description

Native Porcine Porcine IgG, Fab

Source: Normal serum (Accession #:6912366)

Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Purity

Multi-step

Application Notes

This product has been tested by SDS-Page and are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.

Protein / Peptide Type

Native Protein

Formulation, Preparation, and Storage

NBP1-97010

Preparation Method	This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Swine Serum, anti-Swine IgG and anti-Swine IgG F(ab')2. No reaction was observed against anti-Swine IgG F(c) or anti- Papain.
Formulation	0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative	0.01% Sodium Azide
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.

Background: Porcine IgG, Fab

Swine IgG Fab Fragment - secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The F(ab) fragment is the portion of the antibody that binds to the antigen target. The immunoglobulin Fab also possesses one constant and one variable region of both the heavy and light chain.

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Product Specific Notices for Porcine IgG, Fab Native Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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