Porimin Antibody (CL14381) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44335

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL14381

Format

Azide and BSA Free
View all Porimin Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of Q8N131, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Porimin Antibody (CL14381) - Azide and BSA Free

Porimin Antibody (CL14381) - Azide and BSA Free Immunohistochemistry-Paraffin: Porimin Antibody [NBP3-44335]

Immunohistochemistry-Paraffin: Porimin Antibody [NBP3-44335]

Analysis in human tonsil and skeletal muscle tissues using NBP3-44335 antibody. Corresponding Porimin RNA-seq data are presented for the same tissues.
Porimin Antibody (CL14381) - Azide and BSA Free Immunohistochemistry-Paraffin: Porimin Antibody [NBP3-44335]

Immunohistochemistry-Paraffin: Porimin Antibody [NBP3-44335]

Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Porimin Antibody (CL14381) - Azide and BSA Free Immunohistochemistry-Paraffin: Porimin Antibody [NBP3-44335]

Immunohistochemistry-Paraffin: Porimin Antibody [NBP3-44335]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
Porimin Antibody (CL14381) - Azide and BSA Free Immunohistochemistry-Paraffin: Porimin Antibody [NBP3-44335]

Immunohistochemistry-Paraffin: Porimin Antibody [NBP3-44335]

Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
Porimin Antibody (CL14381) - Azide and BSA Free Immunohistochemistry-Paraffin: Porimin Antibody [NBP3-44335]

Immunohistochemistry-Paraffin: Porimin Antibody [NBP3-44335]

Staining of human tonsil shows moderate to strong cytoplasmic positivity in germinal center cells.

Applications for Porimin Antibody (CL14381) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Porimin

Porimin was identified by a monoclonal antibody developed by immunizing mice with apoptotic cells. One of these antibodies, designated anti-Porimin (for pro-oncosis receptor inducing membrane injury), was found to directly induce a unique type of cell death in Jurkat cells by binding to a 110-kDa cell surface receptor on Jurkat cells. Anti-Porimin-mediated cell death was preceded by cell aggregation, formation of plasma membrane pores, and the appearance of membrane blebs. More important, these cells show neither DNA fragmentation nor apoptotic bodies, but display lethal damage of the cell membrane.

Long Name

Pro-Oncosis Receptor Inducing Membrane Injury

Alternate Names

KCT3, TMEM123

Gene Symbol

TMEM123

Additional Porimin Products

Product Documents for Porimin Antibody (CL14381) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Porimin Antibody (CL14381) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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