Recombinant E. coli PPIL1 Protein

Name: Recombinant E. coli PPIL1 Protein
Brand: Novus Biologicals
SKU: NBC1-18375
Price: 419 USD
Availability: InStock

Novus Biologicals | Catalog # NBC1-18375

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Applications

Enzyme Activity, SDS-PAGE

View all PPIL1 Proteins and Enzymes »

Product Specifications

Description

An un-tagged recombinant protein corresponding to the amino acids1-196 of E.coli PPIL1

Source: E.coli

Amino Acid Sequence: MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli PPIL1 Protein

SDS-PAGE: Recombinant E. coli PPIL1 Protein [NBC1-18375]

SDS-Page: PPIL1 Protein [NBC1-18375] - SlyD, 21 kDa (196 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Formulation, Preparation, and Storage

NBC1-18375

Formulation	20 mM Tris-HCl buffer (pH 7.5)
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPIL1

SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase. Recombinant E.Coli slyD was expressed in E.coli and purified by conventional chromatography.

Alternate Names

CYPL1cyclophilin-related gene 1, EC 5.2.1.8, hCyPX, MGC678, peptidyl-prolyl cis-trans isomerase, peptidyl-prolyl cis-trans isomerase-like 1, peptidylprolyl isomerase (cyclophilin)-like 1, PPIase, Rotamase PPIL1

Gene Symbol

PPIL1

Additional PPIL1 Products

Product Documents for Recombinant E. coli PPIL1 Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant E. coli PPIL1 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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