Recombinant Human PPIL4 His Protein

Novus Biologicals | Catalog # NBP1-50877

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

Enzyme Activity, SDS-PAGE
View all PPIL4 Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-492 of Human PPIL4

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAVLLETTLG DVVIDLYTEE RPRACLNFLK LCKIKYYNYC LIHNVQRDFI IQTGDPTGTG RGGESIFGQL YGDQASFFEA EKVPRIKHKK KGTVSMVNNG SDQHGSQFLI TTGENLDYLD GVHTVFGEVT EGMDIIKKIN ETFVDKDFVP YQDIRINHTV ILDDPFDDPP DLLIPDRSPE PTREQLDSGR IGADEEIDDF KGRSAEEVEE IKAEKEAKTQ AILLEMVGDL PDADIKPPEN VLFVCKLNPV TTDEDLEIIF SRFGPIRSCE VIRDWKTGES LCYAFIEFEK EEDCEKAFFK MDNVLIDDRR IHVDFSQSVA KVKWKGKGGK YTKSDFKEYE KEQDKPPNLV LKDKVKPKQD TKYDLILDEQ AEDSKSSHSH TSKKHKKKTH HCSEEKEDED YMPIKNTNQD IYREMGFGHY EEEESCWEKQ KSEKRDRTQN RSRSRSRERD GHYSNSHKSK YQTDLYERER SKKRDRSRSP KKSKDKEKSK YR

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

59.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 190 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PPIL4 His Protein

SDS-PAGE: Recombinant Human PPIL4 His Protein [NBP1-50877]

SDS-PAGE: Recombinant Human PPIL4 His Protein [NBP1-50877]

SDS-Page: Recombinant Human PPIL4 Protein [NBP1-50877] - 15 % SDS-PAGE (3ug)

Formulation, Preparation, and Storage

NBP1-50877
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2 mM DTT, 0.1 M NaCl
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPIL4

PPIL4, also known as HDCME13P, is evolutionarily conserved member of the cyclophilin-type PPIase family of proteins. Ubiquitously expressed with predominant expression in kidney, PPIL4 localizes to the nucleus and contains one PPIase cyclophilin-type domain, a lysine-rich domain, a pair of bipartite nuclear targeting sequences and one RRM (RNA recognition motif) domain. The presence of the RRM domain along with nuclear targeting sequences suggests that PPIL4 may be involved in transcriptional regulation. Recombinant human PPIL4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

Cyclophilin-like protein PPIL4, cyclophilin-type peptidyl-prolyl cis-trans isomerase, EC 5.2.1.8, HDCME13P, peptidyl-prolyl cis-trans isomerase-like 4, peptidylprolyl isomerase (cyclophilin)-like 4, PPIase, Rotamase PPIL4, serologically defined breast cancer antigen NY-BR-18

Gene Symbol

PPIL4

Additional PPIL4 Products

Product Documents for Recombinant Human PPIL4 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human PPIL4 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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