PPM1D Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-12941

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (95%), Chimpanzee (100%), Mouse (96%), Rat (96%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PPM1D Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPM1D. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for PPM1D Antibody - BSA Free

Western Blot: PPM1D Antibody [NBP3-12941]

Western Blot: PPM1D Antibody [NBP3-12941]

Western Blot: PPM1D Antibody [NBP3-12941] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PPM1D antibody (NBP3-12941) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: PPM1D Antibody [NBP3-12941]

Immunocytochemistry/ Immunofluorescence: PPM1D Antibody [NBP3-12941]

Immunocytochemistry/Immunofluorescence: PPM1D Antibody [NBP3-12941] - PPM1D antibody detects PPM1D protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PPM1D stained by PPM1D antibody (NBP3-12941) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.
Western Blot: PPM1D Antibody [NBP3-12941]

Western Blot: PPM1D Antibody [NBP3-12941]

Western Blot: PPM1D Antibody [NBP3-12941] - Jurkat whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PPM1D antibody (NBP3-12941) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: PPM1D Antibody [NBP3-12941]

Western Blot: PPM1D Antibody [NBP3-12941]

Western Blot: PPM1D Antibody [NBP3-12941] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PPM1D antibody (NBP3-12941) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: PPM1D Antibody [NBP3-12941]

Western Blot: PPM1D Antibody [NBP3-12941]

Western Blot: PPM1D Antibody [NBP3-12941] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PPM1D antibody (NBP3-12941) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for PPM1D Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:10000
Application Notes
ICC/IF-Assay dependent

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPM1D

PPM1D is encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development.

Long Name

Protein Phosphatase, Mg2+/Mn2+ Dependent, 1D

Alternate Names

PP2C-delta, Wip1

Gene Symbol

PPM1D

Additional PPM1D Products

Product Documents for PPM1D Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PPM1D Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Phosphatases and Regulators

Customer Reviews for PPM1D Antibody - BSA Free

There are currently no reviews for this product. Be the first to review PPM1D Antibody - BSA Free and earn rewards!

Have you used PPM1D Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies