PPME1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-38515
![Western Blot: PPME1 Antibody [NBP2-38515]](https://resources.rndsystems.com/images/products/PPME1-Antibody-Western-Blot-NBP2-38515-img0005.jpg "Western Blot: PPME1 Antibody [NBP2-38515]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (99%), Rat (99%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: PPLPGSGGSQSGAKMRMGPGRKRDFSPVPWSQYFESMEDVEVENETGKDTFRVYKSGSEGPVLLLLHGGG
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PPME1 Antibody - BSA Free
Western Blot: PPME1 Antibody [NBP2-38515]
Western Blot: PPME1 Antibody [NBP2-38515] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line CACO-2![Immunohistochemistry-Paraffin: PPME1 Antibody [NBP2-38515]](https://resources.rndsystems.com/images/products/PPME1-Antibody-Immunohistochemistry-Paraffin-NBP2-38515-img0004.jpg "Immunohistochemistry-Paraffin: PPME1 Antibody [NBP2-38515]")
Immunohistochemistry-Paraffin: PPME1 Antibody [NBP2-38515]
Immunohistochemistry-Paraffin: PPME1 Antibody [NBP2-38515] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.![Simple Western: PPME1 Antibody [NBP2-38515]](https://resources.rndsystems.com/images/products/PPME1-Antibody-Simple-Western-NBP2-38515-img0002.jpg "Simple Western: PPME1 Antibody [NBP2-38515]")
Simple Western: PPME1 Antibody [NBP2-38515]
Simple Western: PPME1 Antibody [NBP2-38515] - Simple Western lane view shows a specific band for PPME1 in 0.2 mg/ml of HT-29 (left) and HCT 116 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation systems.![Simple Western: PPME1 Antibody [NBP2-38515]](https://resources.rndsystems.com/images/products/PPME1-Antibody-Simple-Western-NBP2-38515-img0003.jpg "Simple Western: PPME1 Antibody [NBP2-38515]")
Simple Western: PPME1 Antibody [NBP2-38515]
Simple Western: PPME1 Antibody [NBP2-38515] - Electropherogram image of the corresponding Simple Western lane view. PPME1 antibody was used at 1:25 dilution on HT-29 and HCT 116 lysate(s) respectively.Applications for PPME1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Simple Western
1:25
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Simple Western: PPME1 antibody was used at 1:25 dilution.
See Simple Western Antibody Database for Simple Western validation: Tested in HT-29 and HCT 116 lysates, separated by Size, antibody dilution of 1:25
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PPME1
Alternate Names
EC 3.1.1, EC 3.1.1.-, FLJ22226, PME-1PME1, protein phosphatase methylesterase 1, protein phosphatase methylesterase-1
Gene Symbol
PPME1
UniProt
Additional PPME1 Products
Product Documents for PPME1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PPME1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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