PPP2R2D Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-15081
![Western Blot: PPP2R2D Antibody [NBP2-15081]](https://resources.rndsystems.com/images/products/PPP2R2D-Antibody-Western-Blot-NBP2-15081-img0004.jpg "Western Blot: PPP2R2D Antibody [NBP2-15081]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Chicken (98%), Xenopus (95%), Zebrafish (95%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human PPP2R2D. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (95%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PPP2R2D Antibody - BSA Free
Western Blot: PPP2R2D Antibody [NBP2-15081]
Western Blot: PPP2R2D Antibody [NBP2-15081] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP2R2D antibody [N2C3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Immunohistochemistry-Paraffin: PPP2R2D Antibody [NBP2-15081]](https://resources.rndsystems.com/images/products/PPP2R2D-Antibody-Immunohistochemistry-Paraffin-NBP2-15081-img0007.jpg "Immunohistochemistry-Paraffin: PPP2R2D Antibody [NBP2-15081]")
Immunohistochemistry-Paraffin: PPP2R2D Antibody [NBP2-15081]
Immunohistochemistry-Paraffin: PPP2R2D Antibody [NBP2-15081] - Rat testis. PPP2R2D antibody [N2C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.![Immunohistochemistry-Paraffin: PPP2R2D Antibody [NBP2-15081]](https://resources.rndsystems.com/images/products/PPP2R2D-Antibody-Immunohistochemistry-Paraffin-NBP2-15081-img0005.jpg "Immunohistochemistry-Paraffin: PPP2R2D Antibody [NBP2-15081]")
Immunohistochemistry-Paraffin: PPP2R2D Antibody [NBP2-15081]
Immunohistochemistry-Paraffin: PPP2R2D Antibody [NBP2-15081] - Mouse testis. PPP2R2D antibody [N2C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.![Immunohistochemistry-Paraffin: PPP2R2D Antibody [NBP2-15081]](https://resources.rndsystems.com/images/products/PPP2R2D-Antibody-Immunohistochemistry-Paraffin-NBP2-15081-img0006.jpg "Immunohistochemistry-Paraffin: PPP2R2D Antibody [NBP2-15081]")
Immunohistochemistry-Paraffin: PPP2R2D Antibody [NBP2-15081]
Immunohistochemistry-Paraffin: PPP2R2D Antibody [NBP2-15081] - Mouse duodenum. PPP2R2D antibody [N2C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: PPP2R2D Antibody [NBP2-15081] -
Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP2R2D antibody [N2C3] (NBP2-15081) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.Applications for PPP2R2D Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:10000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PPP2R2D
Alternate Names
KIAA1541, MDS026, PP2A subunit B isoform B55-delta, PP2A subunit B isoform delta, PP2A subunit B isoform PR55-delta, PP2A subunit B isoform R2-delta, protein phosphatase 2, regulatory subunit B, delta, protein phosphatase 2, regulatory subunit B, delta isoform, serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B deltaisoform
Gene Symbol
PPP2R2D
UniProt
Additional PPP2R2D Products
Product Documents for PPP2R2D Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PPP2R2D Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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