PRAK Antibody (OTI5E1) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-73594

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI5E1

Format

Azide and BSA Free
View all PRAK Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 197-471 of human MAPKAPK5 (NP_003659) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for PRAK Antibody (OTI5E1) - Azide and BSA Free

Western Blot: PRAK Antibody (OTI5E1)Azide and BSA Free [NBP2-73594]

Western Blot: PRAK Antibody (OTI5E1)Azide and BSA Free [NBP2-73594]

Western Blot: PRAK Antibody (OTI5E1) - Azide and BSA Free [NBP2-73594] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRAK.

Applications for PRAK Antibody (OTI5E1) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PRAK

PRAK is encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported.

Alternate Names

EC 2.7.11, MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK-5, mitogen-activated protein kinase-activated protein kinase 5, p38-regulated/activated protein kinase, PRAKEC 2.7.11.1

Gene Symbol

MAPKAPK5

Additional PRAK Products

Product Documents for PRAK Antibody (OTI5E1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PRAK Antibody (OTI5E1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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