Human Prekallikrein ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-60480

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell Culture, Saliva, Milk, CSF (50 ul)

Sensitivity

1.2 ng/ml (example only; lot dependent)

Assay Range

1.25 - 80 ng/mL (example only; lot dependent)
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Description

This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.80% (example only; lot dependent)

Inter-Assay Precision (Precision between assays) CV% 9.80% (example only; lot dependent)

Recovery for Human Prekallikrein ELISA Kit (Colorimetric)

Recovery

97.00% (example only; lot dependent)

Scientific Data Images for Human Prekallikrein ELISA Kit (Colorimetric)

ELISA: Human Prekallikrein ELISA Kit (Colorimetric) [NBP2-60480] -

Sample standard curve

Kit Contents for Human Prekallikrein ELISA Kit (Colorimetric)

  • Biotinylated Human Prekallikrein Antibody (50x)
  • Chromogen Substrate (1x)
  • Human Prekallikrein Microplate
  • Human Prekallikrein Standard
  • MIX Diluent Concentrate (10x)
  • Sealing Tapes:
  • SP Conjugate (100x)
  • Stop Solution (1x)
  • Wash Buffer Concentrate (20x)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Prekallikrein

This gene encodes a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. The encoded preproprotein present in plasma as a non-covalent complex with high molecular weight kininogen undergoes proteolytic processing mediated by activated coagulation factor XII to generate a disulfide-linked, heterodimeric serine protease comprised of heavy and light chains. Certain mutations in this gene cause prekallikrein deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]

Alternate Names

kallikrein B, plasma (Fletcher factor) 1, kininogenin, KLK3, PKK, PKKD, plasma kallikrein, Plasma prekallikrein, PPK

Gene Symbol

KLKB1

Additional Prekallikrein Products

Product Documents for Human Prekallikrein ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human Prekallikrein ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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